EpiSeeker Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)
Overview
- Product nameEpiSeeker Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric)See all Histone H4 (K20 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity5 ng/well
- Range20 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.
EpiSeeker Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097) enables the user to measure global tri-methylation of histone H4-K20. The global H4-K20 tri-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global tri-methyl histone H4-K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115097 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESC1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESC2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESC3 (Detecting Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (Color Developer) 1 x 5ml 1 x 10ml ESC5 (Stop Solution) 1 x 3ml 1 x 6ml Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)
ab115097 has not yet been referenced specifically in any publications.
