For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.
EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461) is designed for the easy and fast colorimetric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
Store EJM3, EJM4 and EJM6 at –20°C away from light.
Store EJM1, EJM5, EJM7, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EJM2, EJM8, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Co-factor 1||1 x 30µl||1 x 60µl|
|Co-factor 2||1 x 30µl||1 x 60µl|
|Co-factor 3||1 x 30µl||1 x 60µl|
|EJM1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EJM2 (JMJD2 Assay Buffer)||1 x 4ml||1 x 28ml|
|EJM3 (JMJD2 Substrate, 50 µg/ml)||1 x 60µl||1 x 120µl|
|EJM4 (JMJD2 Assay Standard, 50 µg/ml)||1 x 10µl||1 x 20µl|
|EJM5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EJM6 (Detection Antibody, 400 µg/ml)||1 x 6µl||1 x 12µl|
|EJM7 (Developer Solution)||1 x 5ml||1 x 10ml|
|EMJ8 (Stop Solution)||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab113461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113461 has not yet been referenced specifically in any publications.
Publishing research using ab113461 ? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"