EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
Overview
- Product nameEpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric)See all KDM4/JMJD2 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.
EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462) is designed for the easy and fast fluorometric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
- Notes
Store EJM3, EJM4, EJM6 and EJM7 at –20°C away from light.
Store EJM1, EJM5, EJM8, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EJM2, EJM9, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl EJM1 (10X Wash Buffer) 1 x 14ml 1 x 28ml EJM2 (JMJD2 Assay Buffer) 1 x 4ml 1 x 8ml EJM3 (JMJD2 Substrate, 50 µg/ml) 1 x 60µl 1 x 120µl EJM4 (JMJD2 Assay Standard, 50 µg/ml) 1 x 10µl 1 x 20µl EJM5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EJM6 (Detection Antibody, 400 µg/ml) 1 x 6µl 1 x 12µl EJM7 (Fluoro-Developer) 1 x 10µl 1 x 20µl EJM8 (Fluoro-Enhancer) 1 x 10µl 1 x 20µl EJM9 (Fluoro-Dilution) 1 x 4ml 1 x 8ml -
Research Areas
Applications
Our Abpromise guarantee covers the use of ab113462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) images
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Functional Studies - EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113462.
Protocols
References for EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
ab113462 has not yet been referenced specifically in any publications.
