EpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
Overview
- Product nameEpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric)See all KMD5/JARID kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.
EpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
- Notes
Store EJA3, EJA4, EJA6 and EJA7 at –20°C away from light.
Store EJA1, EJA5, EJA8, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EJA2, EJA9, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl EJA1 (10X Wash Buffer) 1 x 14ml 1 x 28ml EJA2 (JARID Assay Buffer) 1 x 4ml 1 x 8ml EJA3 (JARID Substrate, 50 µg/ml) 1 x 60µl 1 x 120µl EJA4 (JARID Assay Standard, 50 µg/ml) 1 x 10µl 1 x 20µl EJA5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EJA6 (Detection Antibody, 400 µg/ml) 1 x 6µl 1 x 12µl EJA7 (Fluoro Developer) 1 x 10µl 1 x 20µl EJA8 (Fluoro Enhancer) 1 x 10µl 1 x 20µl EJA9 (Fluoro Dilution) 1 x 4ml 1 x 8ml -
Research Areas
Applications
Our Abpromise guarantee covers the use of ab113464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric) images
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Functional Studies - EpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464
Protocols
References for EpiSeeker KMD5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
ab113464 has not yet been referenced specifically in any publications.
