EpiSeeker MBD2 Binding Activity Quantification Assay Kit (ab113473)
Overview
- Product nameEpiSeeker MBD2 Binding Activity Quantification Assay KitSee all MBD2 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
MBD2 (methyl-CpG binding domain protein 2) belongs to a family of MBD domain containing proteins. This family of proteins associates with heterochromatin by their interaction with methylated DNA at CpG islands, and appear to 'read' and 'translate' the DNA methylation signal into transcriptional repression by recruiting silencing complexes and histone deacetylases among other proteins. MBD2 is the methyl-binding component of the MeCP1 complex, which allows MBD2 to target HDAC/chromatin remodeling activity to methylated templates.
EpiSeeker MBD2 Binding Activity Quantification Assay Kit (ab113473) allows the user to measure binding activity of MBD2 to methylated DNA in human cells/tissues. It can also be used to determine inhibitors of MBD2 activity.
- Notes
Store EMC5 at –20°C away from light.
Store EMC1, EMC3, EMC4, EMC6, and 8-Well Assay Strips at 4°C away from light.
Store all other components (EMC2, EMC7) at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EMC1 (10X Wash Buffer) 1 x 12ml 1 x 25ml EMC2 (Assay Buffer) 1 x 1.8ml 1 x 4ml EMC3 (Methylated DNA, 25 µg/ml) 1 x 50µl 1 x 100µl EMC4 (Affinity Antibody, 100 µg/ml) 1 x 25µl 1 x 50µl EMC5 (Detection Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl EMC6 (Developing Solution) 1 x 6ml 1 x 12ml EMC7 (Stop Solution) 1 x 3ml 1 x 6ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker MBD2 Binding Activity Quantification Assay Kit (ab113473)
ab113473 has not yet been referenced specifically in any publications.
