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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
MBD2 (methyl-CpG binding domain protein 2) belongs to a family of MBD domain containing proteins. This family of proteins associates with heterochromatin by their interaction with methylated DNA at CpG islands, and appear to 'read' and 'translate' the DNA methylation signal into transcriptional repression by recruiting silencing complexes and histone deacetylases among other proteins. MBD2 is the methyl-binding component of the MeCP1 complex, which allows MBD2 to target HDAC/chromatin remodeling activity to methylated templates.
EpiSeeker MBD2 Binding Activity Quantification Assay Kit (ab113473) allows the user to measure binding activity of MBD2 to methylated DNA in human cells/tissues. It can also be used to determine inhibitors of MBD2 activity.
Store EMC5 at –20°C away from light.
Store EMC1, EMC3, EMC4, EMC6, and 8-Well Assay Strips at 4°C away from light.
Store all other components (EMC2, EMC7) at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EMC1 (10X Wash Buffer)||1 x 12ml||1 x 25ml|
|EMC2 (Assay Buffer)||1 x 1.8ml||1 x 4ml|
|EMC3 (Methylated DNA, 25 µg/ml)||1 x 50µl||1 x 100µl|
|EMC4 (Affinity Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EMC5 (Detection Antibody, 200 µg/ml)||1 x 10µl||1 x 20µl|
|EMC6 (Developing Solution)||1 x 6ml||1 x 12ml|
|EMC7 (Stop Solution)||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113473 has not yet been referenced specifically in any publications.
Publishing research using ab113473 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"