EpiSeeker Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477)
Overview
- Product nameEpiSeeker Nuclear (Nucleic Acid-Free) Extraction Kit
- Tests1 x 100 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time1h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
EpiSeeker Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
ab113477 can be used to extract nuclear proteins from mammalian cells and tissue samples free of nucleic acids attached.
Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.
- Notes
Store ENN3 and ENN4 at –20°C in aliquots.
Store all other components at 4°C.
The kit is stable for up to 1 year from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 100 tests 1000x DTT Solution 1 x 100µl 1000X Protease Inhibitor Cocktail (PIC) 1 x 100µl ENN1 (10X Pre-Extraction Buffer) 1 x 10ml ENN2 (Extraction Buffer) 1 x 10ml ENN3 (Extraction Pre-Cleaner) 1 x 1ml ENN4 (Extraction Cleaner) 1 x 100µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477)
ab113477 has not yet been referenced specifically in any publications.
