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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
EpiSeeker hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) enables the user to enrich hydroxymethated DNA using an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA hMeDIP. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.
Store EMH7 at –20°C away from light.
Store EMH1, EMH5, EMH6, EMH8, EMH9, EMH10, and 8-Well Assay Strips at 4°C away from light.
Store remaining components at room temperature away from light.
|Components||24 tests||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units||12 units|
|Adhesive 8-Well Strip Film||3 units||6 units||12 units|
|EMH1 (10X Wash Buffer)||1 x 5ml||1 x 10ml||1 x 20ml|
|EMH10 (Control Primer-Reverse, 20 µM)||1 x 5µl||1 x 10µl||1 x 20µl|
|EMH2 (Antibody Buffer)||1 x 4ml||1 x 8ml||1 x 16ml|
|EMH3 (hMeDIP Solution)||1 x 3ml||1 x 6ml||1 x 12ml|
|EMH4 (DNA Release Buffer)||1 x 7ml||1 x 14ml||1 x 28ml|
|EMH5 (Non-Immune IgG ,0.6 mg/ml)||1 x 10µl||1 x 20µl||1 x 40µl|
|EMH6 (5-hmC Antibody, 0.6 mg/ml)||1 x 25µl||1 x 50µl||1 x 100µl|
|EMH7 (Control DNA, 500 ng/ml)||1 x 5µl||1 x 10µl||1 x 20µl|
|EMH8 (Proteinase K, 10 mg/ml)||1 x 28µl||1 x 56µl||1 x 112µl|
|EMH9 (Control Primer-Forward, 20 µM)||1 x 5µl||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab117134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117134 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"