EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)

Overview

  • Product nameEpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric)See all hydroxymethylated DNA kits ...
  • Sample type
    Saliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
  • Sensitivity
    >= 10 pg/well
  • Assay time
    3h 20m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Plants, all Mammals, Fungi
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

    EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.

    It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

  • Notes

    Store ESD3, ESD4, ESD5, ESD7, ESD8 and ESD9 at –20°C away from light

    Store ESD1, ESD6, ESD10, and the 8-Well Assay Strips at 4°C away from light

    Store ESD2 and ESD11 at room temperature away from light.

    All components of the kit are stable for 6 months from the date of shipment, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 48 tests 96 tests
    8-Well Assay Strips (with Frame) 6 units 12 units
    ESD1 (10X Wash Buffer) 1 x 14ml 1 x 28ml
    ESD10 (Fluoro Enhancer) 1 x 8µl 1 x 16µl
    ESD11 (Fluoro Dilutor) 1 x 4ml 1 x 8ml
    ESD2 (Binding Solution) 1 x 5ml 1 x 10ml
    ESD3 (Negative Control I, 20 µg/ml) 1 x 10µl 1 x 20µl
    ESD4 (Negative Control II, 20 µg/ml) 1 x 10µl 1 x 20µl
    ESD5 (Positive Control, 20 µg/ml) 1 x 10µl 1 x 20µl
    ESD6 (Capture Antibody, 1000 µg/ml) 1 x 4µl 1 x 8µl
    ESD7 (Detection Antibody, 400 µg/ml) 1 x 8µl 1 x 16µl
    ESD8 (Enhancer Solution) 1 x 8µl 1 x 16µl
    ESD9 (Fluoro Developer) 1 x 8µl 1 x 16µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117131 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) images

    • Demonstration of high sensitivity and specificity of 5-hydroxymethylcytosine detection achieved using ab117131. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117131.

    Protocols

    References for EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)

    ab117131 has not yet been referenced specifically in any publications.

    Product Wall

    Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

    Read More

    Thank you for contacting Abcam regarding our EpiSeeker Hydroxymethylated DNA Quantification kits.


    The main difference between the colorimetric and fluorometric kits is the method of detection - this will require either a microplate read...

    Read More


    I would like to inform you that we are still investigating the problem you experienced with the kits. The lab has already provided the following information: "The reading can be carried out at 530/590 nm +/- 10 generally. Thus, emission at 59...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"