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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
Store ESD3, ESD4, ESD5, ESD7, ESD8 and ESD9 at –20°C away from light
Store ESD1, ESD6, ESD10, and the 8-Well Assay Strips at 4°C away from light
Store ESD2 and ESD11 at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|ESD1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|ESD10 (Fluoro Enhancer)||1 x 8µl||1 x 16µl|
|ESD11 (Fluoro Dilutor)||1 x 4ml||1 x 8ml|
|ESD2 (Binding Solution)||1 x 5ml||1 x 10ml|
|ESD3 (Negative Control I, 20 µg/ml)||1 x 10µl||1 x 20µl|
|ESD4 (Negative Control II, 20 µg/ml)||1 x 10µl||1 x 20µl|
|ESD5 (Positive Control, 20 µg/ml)||1 x 10µl||1 x 20µl|
|ESD6 (Capture Antibody, 1000 µg/ml)||1 x 4µl||1 x 8µl|
|ESD7 (Detection Antibody, 400 µg/ml)||1 x 8µl||1 x 16µl|
|ESD8 (Enhancer Solution)||1 x 8µl||1 x 16µl|
|ESD9 (Fluoro Developer)||1 x 8µl||1 x 16µl|
Our Abpromise guarantee covers the use of ab117131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117131 has not yet been referenced specifically in any publications.
Publishing research using ab117131 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"