EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)
Overview
- Product nameEpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric)See all hydroxymethylated DNA kits ...
- Sample typeSaliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
- Sensitivity>= 10 pg/well
- Assay time3h 20m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Plants, all Mammals, Fungi - Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
- Notes
Store ESD3, ESD4, ESD5, ESD7, ESD8 and ESD9 at –20°C away from light
Store ESD1, ESD6, ESD10, and the 8-Well Assay Strips at 4°C away from light
Store ESD2 and ESD11 at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units ESD1 (10X Wash Buffer) 1 x 14ml 1 x 28ml ESD10 (Fluoro Enhancer) 1 x 8µl 1 x 16µl ESD11 (Fluoro Dilutor) 1 x 4ml 1 x 8ml ESD2 (Binding Solution) 1 x 5ml 1 x 10ml ESD3 (Negative Control I, 20 µg/ml) 1 x 10µl 1 x 20µl ESD4 (Negative Control II, 20 µg/ml) 1 x 10µl 1 x 20µl ESD5 (Positive Control, 20 µg/ml) 1 x 10µl 1 x 20µl ESD6 (Capture Antibody, 1000 µg/ml) 1 x 4µl 1 x 8µl ESD7 (Detection Antibody, 400 µg/ml) 1 x 8µl 1 x 16µl ESD8 (Enhancer Solution) 1 x 8µl 1 x 16µl ESD9 (Fluoro Developer) 1 x 8µl 1 x 16µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) images
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Functional Studies - EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)Demonstration of high sensitivity and specificity of 5-hydroxymethylcytosine detection achieved using ab117131. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117131.
Protocols
References for EpiSeeker hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)
ab117131 has not yet been referenced specifically in any publications.
