EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)

Overview

  • Product nameEpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP KitSee all 5-methylcytosine kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

    EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

  • Notes

    Store Normal Mouse IgG, Proteinase K, Anti-5-Methylcytosine and 8-Well Assay Strips at 4°C.

    Store all other components all other components at room temperature.

    The kit can be stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-5-Methylcytosine (1 mg/ml) 1 x 25µl 1 x 50µl
    EMD1 (Wash Buffer) 1 x 28ml 2 x 28ml
    EMD2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    EMD3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml
    EMD3B (Lysis Buffer) 1 x 4ml 1 x 6ml
    EMD4 (ChIP Dilution Buffer) 1 x 4ml 1 x 6ml
    EMD5 (DNA Release Buffer) 1 x 1ml 2 x 2ml
    EMD6 (Reverse Buffer) 1 x 1ml 2 x 2ml
    EMD7 (Binding Buffer) 1 x 5ml 1 x 8ml
    EMD8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)

    ab117135 has not yet been referenced specifically in any publications.

    Product Wall

    Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

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    I apologize again for the incorrect information given originally.

    For tissues we recommend, ab117136.
    In comparison, kit ab117135 would be used when starting with cells. When using a DNA sample, please use ab117133 instead.

    I ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"