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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
Store Normal Mouse IgG, Proteinase K, Anti-5-Methylcytosine and 8-Well Assay Strips at 4°C.
Store all other components all other components at room temperature.
The kit can be stable for up to 6 months from the shipment date, when stored properly.
|Components||24 tests||48 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-5-Methylcytosine (1 mg/ml)||1 x 25µl||1 x 50µl|
|EMD1 (Wash Buffer)||1 x 28ml||2 x 28ml|
|EMD2 (Antibody Buffer)||1 x 15ml||1 x 30ml|
|EMD3A (Pre-Lysis Buffer)||1 x 4ml||1 x 8ml|
|EMD3B (Lysis Buffer)||1 x 4ml||1 x 6ml|
|EMD4 (ChIP Dilution Buffer)||1 x 4ml||1 x 6ml|
|EMD5 (DNA Release Buffer)||1 x 1ml||2 x 2ml|
|EMD6 (Reverse Buffer)||1 x 1ml||2 x 2ml|
|EMD7 (Binding Buffer)||1 x 5ml||1 x 8ml|
|EMD8 (Elution Buffer)||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Normal Mouse IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Proteinase K (10 mg/ml)||1 x 25µl||1 x 50µl|
Our Abpromise guarantee covers the use of ab117135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117135 has not yet been referenced specifically in any publications.
Publishing research using ab117135 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"