EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)
- Product nameEpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP KitSee all 5-methylcytosine kits ...
- Sample typeAdherent cells, Suspension cells
- Assay time4h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
Store Normal Mouse IgG, Proteinase K, Anti-5-Methylcytosine and 8-Well Assay Strips at 4°C.
Store all other components all other components at room temperature.
The kit can be stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-5-Methylcytosine (1 mg/ml) 1 x 25µl 1 x 50µl EMD1 (Wash Buffer) 1 x 28ml 2 x 28ml EMD2 (Antibody Buffer) 1 x 15ml 1 x 30ml EMD3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml EMD3B (Lysis Buffer) 1 x 4ml 1 x 6ml EMD4 (ChIP Dilution Buffer) 1 x 4ml 1 x 6ml EMD5 (DNA Release Buffer) 1 x 1ml 2 x 2ml EMD6 (Reverse Buffer) 1 x 1ml 2 x 2ml EMD7 (Binding Buffer) 1 x 5ml 1 x 8ml EMD8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
- Research Areas
Our Abpromise guarantee covers the use of ab117135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)
ab117135 has not yet been referenced specifically in any publications.