EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)
Overview
- Product nameEpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNASee all 5-methylcytosine kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, a wide range of other species - Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-5-Methylcytosine (1 mg/ml) 1 x 25µl 1 x 50µl EMD1 (Antibody Buffer) 1 x 8ml 1 x 16ml EMD2 (Reaction Buffer) 1 x 4ml 1 x 8ml EMD3 (Wash Buffer) 1 x 16ml 2 x 16ml EMD4 (DNA Release Buffer) 1 x 2ml 1 x 4ml EMD5 (Binding Buffer) 1 x 5ml 1 x 8ml EMD6 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Normal Mouse IgG (1 mg/ml) 1 x 10µl Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl 1 x 20µl Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA images
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Functional Studies - EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)For enrichment of methylated DNA using ab117133, DNA (0.5 ug) isolated from MCF-7 cells was added into the microwell. Methylated DNA was captured by 5-mC antibody prebound to the microwells. -
Functional Studies - EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)Captured methylated DNA was used for analyzing methylation level of GAPDH and MLH1 promoter with the use of primers and probes specific to GAPDH and MLH1 promoters, respectively.
Protocols
References for EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)
ab117133 has not yet been referenced specifically in any publications.
