EpiSeeker methylated DNA Quantification Kit (Colorimetric) (ab117128)

Overview

  • Product nameEpiSeeker methylated DNA Quantification Kit (Colorimetric)See all 5-methylcytosine kits ...
  • Sample type
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Sensitivity
    >= 0.2 ng/well
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    EpiSeeker methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with EpiSeeker hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

  • Notes

    Store ESM3, ESM4, ESM6, and ESM7 at –20°C away from light.

    Store ESM1, ESM5, ESM8, and 8-Well Assay Strips at 4°C away from light.

    Store remaining components (ESM2 and ESM9) at room temperature away from light.

    All components of the kit are stable for 6 months from the date of shipment, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 48 tests 96 tests
    8-Well Assay Strips (with Frame) 6 units 12 units
    ESM1 (10X Wash Buffer) 1 x 14ml 1 x 28ml
    ESM2 (Binding Solution) 1 x 5ml 1 x 10ml
    ESM3 (Negative Control, 20 µg/ml) 1 x 10µl 1 x 20µl
    ESM4 (Positive Control, 20 µg/ml) 1 x 10µl 1 x 20µl
    ESM5 (Capture Antibody, 1000 µg/ml) 1 x 4µl 1 x 8µl
    ESM6 (Detection Antibody, 400 µg/ml) 1 x 8µl 1 x 16µl
    ESM7 (Enhancer Solution) 1 x 8µl 1 x 16µl
    ESM8 (Developer Solution) 1 x 5ml 1 x 10ml
    ESM9 (Stop Solution) 1 x 5ml 1 x 10ml
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117128 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker methylated DNA Quantification Kit (Colorimetric) images

    • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

    Protocols

    References for EpiSeeker methylated DNA Quantification Kit (Colorimetric) (ab117128)

    ab117128 has not yet been referenced specifically in any publications.

    Product Wall


    I am very pleased to hear you would like to accept our offer and test ab117128 with "Flatworm species". This code will give you €350 off your next order before the expiration date. To redeem this offer, please submit an Abreview for...

    Read More

    Thank you for your reply.

    Tween at 0.05 - 0.1% should be used.

    I hope this information helps.

    Thank you for your inquiry.

    I heard back from the lab that generally, PBS-T buffer may be used for washing, however for the best results, they recommend to use the wash buffer included in the kit. We could get the washing buffer (either 14 mL...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"