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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
EpiSeeker methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use with EpiSeeker hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
Store ESM3, ESM4, ESM6, and ESM7 at –20°C away from light.
Store ESM1, ESM5, ESM8, and 8-Well Assay Strips at 4°C away from light.
Store remaining components (ESM2 and ESM9) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|ESM1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|ESM2 (Binding Solution)||1 x 5ml||1 x 10ml|
|ESM3 (Negative Control, 20 µg/ml)||1 x 10µl||1 x 20µl|
|ESM4 (Positive Control, 20 µg/ml)||1 x 10µl||1 x 20µl|
|ESM5 (Capture Antibody, 1000 µg/ml)||1 x 4µl||1 x 8µl|
|ESM6 (Detection Antibody, 400 µg/ml)||1 x 8µl||1 x 16µl|
|ESM7 (Enhancer Solution)||1 x 8µl||1 x 16µl|
|ESM8 (Developer Solution)||1 x 5ml||1 x 10ml|
|ESM9 (Stop Solution)||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab117128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117128 has not yet been referenced specifically in any publications.
Publishing research using ab117128 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"