Overview
- Product nameEpiSeeker methylated control DNA KitSee all DNA modification kits ...
- Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
A methylation-positive control is a must tool for a successful performance of gene methylation studies.
EpiSeeker methylated control DNA Kit (ab117125) provides a methylation-positive control for methylation studies. The kit includes the enzymatically methylated human genomic DNA and all components for DNA modification. Methylated human DNA needs to be modified before it is used as a positive control in MS-PCR. DNA modification reagents included in the kit are sufficient for modifying 5 µg of methylated human genomic DNA (10 modifications).
This product is intended for use with the EpiSeeker DNA Modification Kit series. It can also be used with other DNA modification or methylation kits.
- Notes
Store the Methylated Human Genomic DNA in aliquots at –20°C to avoid multiple freeze-thaws.
Store all other components at room temperature (15-25°C) away from light.
The kit is stable for up to 1 year from date of shipment when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
-
Components 10 tests 20 tests EBM1 (DNA Modifier Powder) 1 x 0.7g 1 x 1.4g EBM2 (DNA Modification Solution) 1 x 1.5ml 1 x 3ml EBM3 (Balance Solution) 1 x 0.08ml 1 x 0.15ml EBM4 (DNA Binding Buffer) 1 x 4ml 1 x 8ml EBM5 (Elution Buffer) 1 x 0.5ml 1 x 1ml Methylated Human Genomic DNA (0.1 mg/ml) 1 x 5µg 1 x 10µg - Research Areas
Applications
Our Abpromise guarantee covers the use of ab117125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker methylated control DNA Kit (ab117125)
ab117125 has not yet been referenced specifically in any publications.
