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Products:Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Anti-Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab82752? Please let us know so that we can cite the reference in this datasheet
ab82752 has been referenced in 1 publications.
- Beer TW et al. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 37:218-23 (2000). IHC-P, IHC-Fr; Human. PubMed: 10971697
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)](/ps/datasheet/images/82/ab82752/Epithelial-Antigen-Primary-antibodies-ab82752-1.jpg)
Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)
ICC/IF image of ab82752 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82752, 1ml) overnight at +4ºC. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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