![Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752) image](#Epithelial-Antigen-Primary-antibodies-ab82752-1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Overview
Product name
Anti-Epithelial Antigen antibody [Ber-EP4], prediluted
See all Epithelial Antigen products (2) ...
Description
Mouse monoclonal [Ber-EP4] to Epithelial Antigen, prediluted
Tested applications
IHC-P, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Human breast carcinoma cell line MCF-7.
Positive control
Kidney.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, Stabilizing agent, pH 7.5
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
Ber-EP4
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
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Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)
![Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752) image](/ps/datasheet/images/82/ab82752/Epithelial-Antigen-Primary-antibodies-ab82752-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab82752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/1(Fix with acetone.)
ICC/IF: Use a concentration of 5 µg/ml
Target
Relevance
Epithelial antigen is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It may may play a role in adhesive functions and in cell to cell interactions, metastasis and signalling and provide a protective layer on epithelial surfaces. It is capable of direct or indirect interaction with actin cytoskeleton.
Cellular localization
Apical cell membrane; Single pass type I membrane protein. Note: Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
Alternative names
- Breast carcinoma associated antigen DF3 antibody
- Carcinoma associated mucin antibody
- CD227 antigen antibody
see all
Database links
- Entrez Gene: 4582 Human
- Omim: 158340 Human
- SwissProt: P15941 Human
Anti-Epithelial Antigen antibody [Ber-EP4], prediluted images:
Immunocytochemistry/ Immunofluorescence - Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)
ICC/IF image of ab82752 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82752, 1ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)
This product has been referenced in:
- Beer TWet al. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 37:218-23 (2000). IHC-P, IHC-Fr; Human.Read more (PubMed: 10971697) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"