Anti-Epoxide hydrolase antibody [15B2AD9] (ab110307)

  Immunocytochemistry/ Immunofluorescence - Epoxide hydrolase antibody [15B2AD9] (ab110307)

Immunocytochemistry image of ab110307-stained Human HDFn cells.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110307 at 4 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue).
Target protein locates mainly in microsomal/ER.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody [15B2AD9] (ab110307)

IHC image of Epoxide hydrolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110307, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times