Anti-ErbB 2 antibody [3B5] (ab16901)

Dear Sir/Madam,
I would like to order the peptide for the epitope of : Mouse monoclonal
[3B5] to ErbB 2, (IgG1) Synthetic peptide: TAENPEYLGLDVPV, corresponding
to C terminal amino acids 1242 - 1255 of Human c-ErbB2 (Ab16901). Is
ErbB 2 peptide (ab38955) the correct one , or do you have the one for
amino acids 1242 - 1255 of Human c-ErbB2? (ab38955: Synthetic peptide
derived from within residues 1200 to the C-terminus of Human ErbB 2.This
peptide was used as an immunogen for ab38956 - ErbB 2 antibody.)
kind regards,

ANSWER:

Thank you for contacting us. The peptide catalog ab38955 does contain the TAENPEYLGLDVPV (aa 1242-1255) sequence, plus a few additional amino acids.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear xxxx
Thanks for your kindly reply, after contacting with this customer and passed these suggestions to him, he indicated this antibody was storage at 4C and he conducted wb many times, therefore he wants to ask for your help to solve this problem as soon as possible, and I also attached his data in this letter, please check it and offer any suggestion to him.

Thanks for your kindly help
Best regards

ANSWER:

Thank you for getting back to me in regards to this complaint.


As discussed previously, the band at ˜170 kDa may well be the specific band for ErbB 2 as the protein goes through post translational modification to increase its weight. In order to reduce the non-specific staining observed the customer could try as previously discussed:


1. to try with a different blocking agent (such as 5%BSA) we would typically recommend incubating with the blocking buffer for 1 hour at 4 degrees. A mild detergent such as Tween 20 (0.2%) in the dilution buffer of the primary antibody may also be beneficial.


2. lowering the concentration of the antibody, (0.1, 0.5 and 1 ug/mL) may improve the results (with incubation at 4 degrees overnight and 1 hour at room temperature if possible). It would also be useful to carry out a no-primary control to see if any of the non-specificity seen is due to the secondary antibody.

If these suggestions have been tried by your customer and they have not seen any improvement the I would be happy to offer a replacement antibody of a different lot, a different antibody against the same target (such as http://www.abcam.com/ErbB-2-antibody-ab2428.htmlwhich is a rabbit polyclonal which has been used with very positive results by many of our customers), or a credit note.


I look forward to hearing how your customer would like to proceed.

I am interested in two antibodies of your company, concretely, ab89171 and ab16901. Because of the total price of them is too high (780 euros), I would be grateful if you could make me an special offer.
I am looking forward to hearing from you.

ANSWER:

Thank you for contacting us.

I am pleased to let you know that all our products are covered by our Abpromise (www.abcam.com/Abpromise):

We guarantee our products work in the tested species and applications as stated on the datasheet.
We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
100% Scientific and Customer Support (now also in Spanish language) for any product you buy from Abcam or one of its authorized distributors.
We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.
I am pleased to inform you of our promotion for which your order would qualify: We do not currently have an image of ab89171 in Western blot or flow cytometry, nor of ab16901 in immunohistochemistry. Therefore, I can offer a discount off a future purchase if you buy ab89171 and/or ab16901 now, use it Western blot/flow cytometry and immunohistochemistry, respectively, and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each, i.e. you could get two free primary antibodies from us.

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and send the images. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

2. Purchase ab89171 and/or ab16901 either by phone, fax, or online (www.abcam.com).

3. Use it in the mentioned applications.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

Thank you for sending us the detail information. It was very helpful. Our customer wanted to ask one additional quesiton. They applied this antibody to MCF7 (human breast cancer cell). They did not  have any good luck with this experiment. Could you please inform us any published paper where ab16901 was successfully used? If you cannot findany, could you recommand any good protocol he can use? FYI, he is treating ab16901 to repress tumeric activity in MCF7 cell. Thank you very much!  

ANSWER:

Thank you for contacting us.

ab16901 was used in 4 publications listed on this link : http://www.abcam.com/ErbB-2-antibody-3B5-ab16901-references.html

To our knowledge none of the anti-ErbB2 antibodies from our catalog has been used in blocking, inhibiting or functionnal assay experiments, so I am afraid that we cannot recommend a specific protocol to this purpose.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product or a protocol that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

Dear Technical team, The customer purchased ab16901 and got several questions. 1. Is ab16901 specific for breast cancer ErbB2? Did you test this antibody against any other cnacer/tissue ErbB2 such as pancreatic cancer, bladder cancer? 2. Could you recommand an ErbB2 antibody that is specific for Human pancreatic cancer ErbB2? Thank you in advance for your help.  

ANSWER:

Thank you for contacting us.

ab16901 is not specific to breast cancer but to ErbB2 protein that is expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

As shown on NCBI (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.446352) the expression of ErbB2 in pancreatic tumor exists but it is not as high as in breast or colorectal tumors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.