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ab2428 has been referenced in 9 publications.
Publishing research using ab2428? Please let us know so that we can cite the reference in this datasheet
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ab2428 at a 1:200 dilution staining SK-BR-3 whole cell lysate.
Western blot of whole cell lysates of cultured cells, used at a concentration of 1:1000, incubated overnight at 4C, and visualized with an HRP conjugated secondary, with a chemilumenescent detection method. Review by Todd Gocken submitted 20 July 2004
ab2428 staining ErbB 2 in human breast cancer, tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking in 10% serum for 1 hour at 20°C.The primary antibody was diluted, 1/100 in TBS and incubated with sample for 12 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary. Left hand-side image show staining with ab2428 and developed using DAB detection system while right hand-side shows Isotype control staining with anti-rabbit IgG and DAB.
This image is courtesy of an anonymous Abreview
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