Anti-ErbB 2 antibody (ab2428)

Any news yet on what mouse tissue was used for staining using this antibody?

ANSWER:

Thank you for your email. I followed up with the lab for you, and the in mouse validation for this antibody was performed using NIH3T3 cells. The IHC validation has been done on human tissues. I hope this helps, please let me know if you need any additional information or assistance.

Wereally appreciate your cooperation,
The purchase order number is EP791427, and Oreder no. 981247.
For themouse monoclonal [33] to estrogen receptor alpha ab2746 the Lot no. GR57110.
Fro m the mouse monoclonal [PR-At 4.14] to PR ab2764 the Lot no. GR38221.
we prefer the same Abs from different lot.

Thank you,

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1073326.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your response, and I appreciate your cooperation
I haveused the same protein extraction for both antibodies (Abcame and Santa-cruz). In section three in the questionnaire I did typo error in writing the concentrations that i havetried in which i repeated the 1/5000 two times. I have used two different concentration first I used1/5000 and then I tried1/500, both did not work. Again I apologize for the mistake inwriting this information in the first email.

Thank you,

ANSWER:

Thank you for confirming the details.

In order to further check, if this issue is lot related; could you provide the Abcam purchase order number or the date when these antibodies were purchased.

Finally, could you let me know if you would like to try same antibodies from different lot or different antibodies against same target as replacements.

Please note the order number EP794778 does not retrieve any information about these product so please provide the correct Abcam order number.

I will look forward to hearing from you soon.

Thank you for your responce.I attached the questionnaire and an image for the blot.

Thanks,

ANSWER:

Thank you for your email. I am sorry to hear that you are experiencing problems with these antibodies.

The protocol looks fine to me, the antibodies should have worked. So my only concern is the sample preparation, which I think could be the reason of antibodies being not working. Could I ask; have you used same sample with ab2428 and Santa Cruz antibodies, that worked?

As protocol troubleshooting I would suggest trying the antibodies at 1/1000 dilution.

Could you please let me know the results after reusing these at low dilutions, I will then process the order for replacement antibodies.

I am contacting you regarding a purchase we made for antibodies. We bought3 antibodies from Abcam (Mouse monoclonal PR-AT 4.14 to progesterone receptor (ab2764), Mouse monoclonal 33 to estrogen receptor alpha (ab2746) and Rabbit polyclonal to ErbB2 (ab2428)). The ErbB2 ab worked well with us in western blot, while both Abs for ER and PR didn't work. I have tried many times and no band detected inapositive control MCF-7 cell lines.
I am wondering if we can receive new Antibodies for the ER-alpha and PR.

Thank you,

ANSWER:

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.