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Western blot - ErbB 2 antibody (ab2428)
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Western blot - ErbB 2 antibody (ab2428)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 2 antibody (ab2428)
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Product Name
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ErbB 2 antibody
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See all ErbB 2 antibodies (39)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ErbB 2
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Immunogen
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Synthetic peptide (Human) (C terminal).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
For Western Blotting, 1:200
Optimal dilutions should be determined by the user.
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Positive control
(see definition)
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SK-BR-3 lysate.
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Cellular localization
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Cell Membrane; single pass type I membrane protein
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ErbB 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ErbB 2 antibody images, references, products related to ab2428 and other tools.
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ErbB 2 antibody images:
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 Western blot - ErbB 2 antibody (ab2428)
ab2428 at a 1:200 dilution staining SK-BR-3 whole cell lysate.
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Western blot - ErbB 2 antibody (ab2428)
Western blot of whole cell lysates of cultured cells, used at a concentration of 1:1000, incubated overnight at 4C, and visualized with an HRP conjugated secondary, with a chemilumenescent detection method. Review by Todd Gocken submitted 20 July 2004
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 2 antibody (ab2428)
ab2428 staining ErbB 2 in human breast cancer, tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking in 10% serum for 1 hour at 20°C.The primary antibody was diluted, 1/100 in TBS and incubated with sample for 12 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary. Left hand-side image show staining with ab2428 and developed using DAB detection system while right hand-side shows Isotype control staining with anti-rabbit IgG and DAB. This image is courtesy of an anonymous Abreview
See Abreview
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References for ErbB 2 antibody (ab2428)
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This product has been used in: (two most recent references)
Abraira VE et al. Vertebrate Lrig3-ErbB interactions occur in vitro but are unlikely to play a role in Lrig3-dependent inner ear morphogenesis. PLoS One 5:e8981 (2010). WB. PubMed: 20126551
Flowers M & Thompson PA t10c12 conjugated linoleic acid suppresses HER2 protein and enhances apoptosis in SKBr3 breast cancer cells: possible role of COX2. PLoS One 4:e5342 (2009). WB. PubMed: 19399184
See all 6 publication references for this product.
If you publish research using ab2428 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ErbB 2
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ErbB 2 antibody - more information
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ErbB 2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ErbB 2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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