Anti-ErbB 2 (phospho Y1221 + Y1222) antibody (ab131102)

Overview

  • Product nameAnti-ErbB 2 (phospho Y1221 + Y1222) antibody
    See all ErbB 2 primary antibodies
  • Description
    Rabbit polyclonal to ErbB 2 (phospho Y1221 + Y1222)
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH derived from around the phosphorylation site of Tyrosine1221/1222 (

    N-L-Y(p)-Y(p)-W

    ) of Human ErbB 2 (UniProt P04626).

  • Positive control
    • MDA cell extract treated or untreated with EGF; MCF cells

Properties

Applications

Our Abpromise guarantee covers the use of ab131102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).
ICC/IF 1/100 - 1/200.

Target

  • FunctionProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • Tissue specificityExpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • Involvement in diseaseHereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
  • Cellular localizationCytoplasm. Nucleus and Cell membrane. Cytoplasm > perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • C erb B2/neu protein antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/NEU antibody
    • HER2 antibody
    • Herstatin antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU proto oncogene antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    see all

Anti-ErbB 2 (phospho Y1221 + Y1222) antibody images

  • All lanes : Anti-ErbB 2 (phospho Y1221 + Y1222) antibody (ab131102) at 1/500 dilution

    Lane 1 : Schwann cell extract untreated
    Lane 2 : Schwann cell extract treated with 10 ng/ml GGF for 30 minutes.

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 137 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Lanes on the left hand side show phosphorylation of ErbB2 at tyrosine 1221 and 1222 (ab131102) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).

    Incubation with 10 ng/ml GGF for 30 minutes does not change the amount of total ErbB2 in rat Schwann cells (as shown on right hand side lanes)

    See Abreview

  • All lanes : Anti-ErbB 2 (phospho Y1221 + Y1222) antibody (ab131102) at 1/500 dilution

    Lane 1 : MDA cell extract untreated
    Lane 2 : MDA cell extract treated with EGF


    Predicted band size : 137 kDa
    Observed band size : 185 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of methanol-fixed MCF cells labelling ErbB 2 (phospho Y1221 + Y1222) with ab131102 at 1/100 dilution.

References for Anti-ErbB 2 (phospho Y1221 + Y1222) antibody (ab131102)

ab131102 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (cultured Schwann cells)
Specification cultured Schwann cells
Treatment 10 ng/ml GGF for 30 min
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Aug 13 2013

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