Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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ab47755 has been referenced in 4 publications.
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All lanes : Anti-ErbB 2 (phospho Y1248) antibody (ab47755) at 1/500 dilution
Lane 1 : SK-OV3 cell extract treated with phosphopeptide
Lane 2 : SK-OV3 cell extract
Predicted band size : 138 kDa
Observed band size : 175 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47755 at a 1/50.
Left image : Un-treated
Right image : Treated with phosphopeptide
ab47755 staining ErbB2 in adult mouse skin tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde, then a heat mediated antigen retrieval step was performed using citric buffer pH 6.0 and blocked with 10% serum for 1 hour at 20ºC followed by incubation with the primary antibody, at a 1/100 dilution, for 12 hours at 4ºC. Abcam biotinylated goat anti-rabbit IgG (H+L) (ready to use) was used as the secondary antibody, catalogue number ab64256.
This image is courtesy of an anonymous abreview.
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