Anti-ErbB 2 (phospho Y1248) antibody (ab47755)

Overview

• Product nameAnti-ErbB 2 (phospho Y1248) antibodySee all ErbB 2 primary antibodies ...
• Description
  Rabbit polyclonal to ErbB 2 (phospho Y1248)
• SpecificityThis antibody is specific for ErbB 2, only when phosphorylated at tyrosine 1248.
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthesized phosphopeptide derived from human ErbB 2 around the phosphorylation site of tyrosine 1248 (P-E-Y^P-L-G)

• Positive controlSK-OV3 cell extract Human breast carcinoma tissue This antibody gave a positive result in IF/ICC when used in the following methanol fixed cell lines: HepG2

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Cancer
  • Growth factors
  • EGF

  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• FunctionProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
• Tissue specificityExpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
• Involvement in diseaseHereditary diffuse gastric cancer (HDGC) [MIM:137215]: A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=The gene represented in this entry is involved in disease pathogenesis.
  Glioma (GLM) [MIM:137800]: Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes. Note=The gene represented in this entry is involved in disease pathogenesis.
  Ovarian cancer (OC) [MIM:167000]: The term ovarian cancer defines malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  Lung cancer (LNCR) [MIM:211980]: A common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis. Note=The gene represented in this entry is involved in disease pathogenesis.
  Gastric cancer (GASC) [MIM:613659]: A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. Note=The gene represented in this entry is involved in disease pathogenesis.
  Note=Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
• Cellular localizationCytoplasm. Nucleus and Cell membrane. Cytoplasm > perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

Target information above from: UniProt accession P04626 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2064 Human
  • Entrez Gene: 13866 Mouse
  • Entrez Gene: 24337 Rat
  • Omim: 164870 Human
  • SwissProt: P04626 Human
  • SwissProt: P70424 Mouse
  • SwissProt: P06494 Rat
  • Unigene: 446352 Human

  • Unigene: 290822 Mouse
  • Unigene: 93966 Rat

  see all

• Alternative names
  Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibodyc erb B2 antibodyC erb B2/neu protein antibody

  C erbB 2 antibodyCD340 antibodyCD340 antigen antibodyCerb B2/neu protein antibodyCerbB2 antibodyCerbB2 antibodyErb B2 antibodyERBB2 antibodyERBB2_HUMAN antibodyHER 2 antibodyHER 2/neu antibodyHER2 antibodyHer2/neu antibodyHerstatin antibodyMetastatic lymph node gene 19 protein antibodyMLN 19 antibodyMLN19 antibodyNEU antibodyNEU Proto Oncogene antibodyNeuro Glioblastoma Derived Oncogene Homolog antibodyNeuroblastoma/glioblastoma derived oncogene homolog antibodyNGL antibodyp185 ErbB2 antibodyp185erbB2 antibodyProto-oncogene c-ErbB-2 antibodyProto-oncogene Neu antibodyReceptor Protein Tyrosine Kinase ErbB2 Precursor antibodyReceptor tyrosine protein kinase erbB 2 antibodyReceptor tyrosine-protein kinase erbB-2 antibodyTKR1 antibodyTyrosine kinase type cell surface receptor HER2 antibodyTyrosine kinase-type cell surface receptor HER2 antibodyV erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibodyv erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibodyV erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibodyVerb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody

  see all

Anti-ErbB 2 (phospho Y1248) antibody images

• 

  Western blot - ErbB 2 (phospho Y1248) antibody (ab47755)
  All lanes : Anti-ErbB 2 (phospho Y1248) antibody (ab47755) at 1/500 dilution
  
  Lane 1 : SK-OV3 cell extract treated with phosphopeptide
  Lane 2 : SK-OV3 cell extract
  
  
  Predicted band size : 138 kDa
  Observed band size : 175 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 2 (phospho Y1248) antibody (ab47755)
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47755 at a 1/50.

  Left image : Un-treated

  Right image : Treated with phosphopeptide

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 2 (phospho Y1248) antibody (ab47755)This image is courtesy of an anonymous abreview.
  ab47755 staining ErbB2 in adult mouse skin tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde, then a heat mediated antigen retrieval step was performed using citric buffer pH 6.0 and blocked with 10% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/100 dilution, for 12 hours at 4°C. Abcam biotinylated goat anti-rabbit IgG (H+L) (ready to use) was used as the secondary antibody, catalogue number ab64256.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ErbB 2 (phospho Y1248) antibody (ab47755)

  ICC/IF image of ab47755 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47755 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.