Anti-ErbB 2 (phospho Y877) antibody (ab47262)

Overview

  • Product nameAnti-ErbB 2 (phospho Y877) antibodySee all ErbB 2 primary antibodies ...
  • Description
    Rabbit polyclonal to ErbB 2 (phospho Y877)
  • SpecificityDetects endogenous levels of ErbB2, only when phosphorylated at Tyrosine 877.
  • Tested applicationsWB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human HER2 around the phosphorylation site of Tyrosine 877 (T-E-YP-H-A).

  • Positive control
    • Human breast carcinoma. EGF-treated MDA-MB-231 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab47262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 138 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • FunctionProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • Tissue specificityExpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • Involvement in diseaseHereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
  • Cellular localizationCytoplasm. Nucleus and Cell membrane. Cytoplasm > perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • c erb B2 antibody
    • C erb B2/neu protein antibody
    • C erbB 2 antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • CerbB2 antibody
    • Erb B2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/neu antibody
    • HER2 antibody
    • Her2/neu antibody
    • Herstatin antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU Proto Oncogene antibody
    • Neuro Glioblastoma Derived Oncogene Homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185 ErbB2 antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody
    • Receptor tyrosine protein kinase erbB 2 antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

Anti-ErbB 2 (phospho Y877) antibody images

  • IHC image of  Angiotensin II Type 1 Receptor staining in Mouse Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47262, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Staining of phosphorilated ErB 2 on breast carcinoma tissue sections using ab47262 at a 1/50 dilution. Right panel: the antibody was pre-incubated with synthesized phosphopeptide.Staining of phosphorilated ErB 2 on breast carcinoma tissue sections using ab47262 at a 1/50 dilution. Right panel: the antibody was pre-incubated with synthesized phosphopeptide.
  • All lanes : Anti-ErbB 2 (phospho Y877) antibody (ab47262) at 1/500 dilution

    Lane 1 : Schwann cell extract untreated
    Lane 2 : Schwann cell extract treated with 10 ng/ml GGF for 30 minutes.

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 138 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Lanes on the left hand side show phosphorylation of ErbB2 at tyrosine 877 (ab47262) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).

     Incubation with 10 ng/ml GGF for 30 minutes does not change the amount of total ErbB2 in rat Schwann cells (as shown on right hand side lanes)

    See Abreview

  • All lanes : Anti-ErbB 2 (phospho Y877) antibody (ab47262) at 1/500 dilution

    Lane 1 : MDA MB 231 cell extract
    Lane 2 : MDA MB 231 cell extract treated with EGF


    Predicted band size : 138 kDa
    Observed band size : 177 kDa (why is the actual band size different from the predicted?)

References for Anti-ErbB 2 (phospho Y877) antibody (ab47262)

This product has been referenced in:
  • Monsey J  et al. Her4 and Her2/neu Tyrosine Kinase Domains Dimerize and Activate in a Reconstituted in Vitro System. J Biol Chem 285:7035-44 (2010). WB . Read more (PubMed: 20022944) »
  • Houshmandi SS  et al. The neurofibromatosis 2 protein, merlin, regulates glial cell growth in an ErbB2- and Src-dependent manner. Mol Cell Biol 29:1472-86 (2009). WB ; Mouse . Read more (PubMed: 19103750) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (cultured Schwann cells)
Specification cultured Schwann cells
Treatment 10 ng/ml GGF for 30 min
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 13 2013

Thanks for your enquiry and for including your completed questionnaire.
I just have a few additional question:
1. Over-fixation can destroy epitopes. 1 month fixation in PFAmay be too long to fix. Typical fixation times are closer to 24 hours...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (nerves)
Loading amount 40 µg
Specification nerves
Gel Running Conditions Reduced Denaturing (4-10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"