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Synthetic phosphopeptide derived from human HER2 around the phosphorylation site of Tyrosine 877 (T-E-YP-H-A).
Our Abpromise guarantee covers the use of ab47262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. Predicted molecular weight: 138 kDa.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
IHC image of Angiotensin II Type 1 Receptor staining in Mouse Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47262, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of an anonymous Abreview
Lanes on the left hand side show phosphorylation of ErbB2 at tyrosine 877 (ab47262) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).
Incubation with 10 ng/ml GGF for 30 minutes does not change the amount of total ErbB2 in rat Schwann cells (as shown on right hand side lanes)
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