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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab20161? Please let us know so that we can cite the reference in this datasheet
ab20161 has been referenced in 1 publications.
- Srinivasan R et al. Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types. J Pathol 185:236-45 (1998). PubMed: 9771476
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - ErbB 3 antibody [RTJ2] (ab20161)](/ps/datasheet/images/20/ab20161/ErbB-3-Primary-antibodies-ab20161-3.jpg)
Immunocytochemistry/ Immunofluorescence - ErbB 3 antibody [RTJ2] (ab20161)
ICC/IF image of ab20161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20161, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - ErbB 3 antibody [RTJ2] (ab20161)](/ps/datasheet/images/20/ab20161/ErbB-3-Primary-antibodies-ab20161-4.jpg)
Flow Cytometry - ErbB 3 antibody [RTJ2] (ab20161)
Overlay histogram showing HeLa cells stained with ab20161 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 3 antibody [RTJ2] (ab20161)](/ps/datasheet/images/20/ab20161/ErbB-3-Primary-antibodies-ab20161-5.jpeg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 3 antibody [RTJ2] (ab20161)
IHC image of ab20161 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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