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All lanes : Anti-ErbB 3 antibody (ab34641) at 5 µl (for immunoprecipitation followed by immunoblot detection
using an anti-phosphotyrosine antibody.
)
Lane 1 : a human LNCap prostate cell line
without the additon of Heregulin ß1
Lane 2 : a human LNCap prostate cell line
treatment with Heregulin ß1 for 15' at 100
ng/ml
developed using the ECL technique
Predicted band size : 148 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
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