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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-ErbB 3 antibody
See all ErbB 3 products (19) ...
Description
Rabbit polyclonal to ErbB 3
Specificity
The antibody is directed against human HER3. experiments.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
HER3 fusion protein: PASEQGYE E MRAFQGPGH QAPHVHYARL KTLRSLEAT D SAF, corresponding to C terminal amino acids 1283-1323 of Human HER3
PASEQGYE E MRAFQGPGH QAPHVHYARL KTLRSL EAT D SAF
Positive control
Human prostate cell lines (LNCap) or human breast cell line (MDA-453)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the storage buffer.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - HER3 antibody (ab34641)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
- 1 Image
WB
Application notes
IP: Use 5µg. The immunoprecipitation mix contained the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).
WB: Use at an assay dependent dilution. In conjunction with a detection antibody like anti-phosphotyrosine. Detects a band of approximately 180 kDa (predicted molecular weight: 148 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds and is activated by neuregulins and NTAK.
Tissue specificity
Epithelial tissues and brain.
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Developmental stage
Overexpressed in a subset of human mammary tumors.
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
Post-translational
modifications
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP21860
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c erbB 3 antibody
- c erbB3 antibody
- ERBB3 antibody
see all
Database links
- Entrez Gene: 2065 Human
- Entrez Gene: 13867 Mouse
- Entrez Gene: 29496 Rat
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q61526 Mouse
- SwissProt: Q62799 Rat
- Unigene: 118681 Human
see all
Anti-ErbB 3 antibody images:
Western blot - HER3 antibody (ab34641)
All lanes : Anti-ErbB 3 antibody (ab34641) at 5 µl (for immunoprecipitation followed by immunoblot detection
using an anti-phosphotyrosine antibody.
)
Lane 1 : a human LNCap prostate cell line
without the additon of Heregulin ß1
Lane 2 : a human LNCap prostate cell line
treatment with Heregulin ß1 for 15' at 100
ng/ml
developed using the ECL technique
Predicted band size : 148 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
References for Anti-ErbB 3 antibody (ab34641)
ab34641 has not yet been referenced specifically in any publications.
Publishing research using ab34641? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"