Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
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Phone call: Customer kindly asked if they can use this antibody in Flow Cytometry. |
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ANSWER: |
DISCOUNT CODE: XXXXXXXX Expiration date: 16-01-2012
I am very pleased to hear you would like to accept our offer and test ab5470 in Flow Cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.
PS: The positive control cell line we can recommend is Jurkat cell line, COLO205 cells or IFN alpha stimulated KAS-6/1, ANBL-6 cell lines. The information about positive control cell lines is easily available in literature so I would recommend searching PubMed. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-ErbB 3 antibody (ab5470) + Mouse brain lysate
Predicted band size : 148 kDa
Observed band size : 155 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HC = hepatocarcinoma.
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