ErbB 3 antibody (ab5470)

Product Name 

ErbB 3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ErbB 3

Immunogen 

Synthetic peptide conjugated to KLH, corresponding to amino acids 41-56 of Human Erb B3.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 148 kDa (predicted molecular weight: 161 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain lysate.

Cellular localization 

Single pass type I membrane protein (isoform 1), Secreted (isoform 2).

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins

Relevance 

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for ErbB 3 antibody images, references, products related to ab5470 and other tools.

ErbB 3 antibody images:

References for ErbB 3 antibody (ab5470)

  This product has been used in:

Yu WM et al. Schwann cell-specific ablation of laminin gamma1 causes apoptosis and prevents proliferation. J Neurosci 25:4463-72 (2005). PubMed: 15872093
If you publish research using ab5470 please let us know so that we can cite the reference on this datasheet.

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