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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Overview
Product name
Anti-ErbB 3 antibody
See all ErbB 3 products (19) ...
Description
Rabbit polyclonal to ErbB 3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 41-56 of Human Erb B3.
Positive control
Mouse brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
Target
Function
Binds and is activated by neuregulins and NTAK.
Tissue specificity
Epithelial tissues and brain.
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Developmental stage
Overexpressed in a subset of human mammary tumors.
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
Post-translational
modifications
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP21860
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c erbB 3 antibody
- c erbB3 antibody
- ERBB3 antibody
see all
Database links
- Entrez Gene: 2065 Human
- Entrez Gene: 13867 Mouse
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q61526 Mouse
- Unigene: 118681 Human
- Unigene: 373043 Mouse
Anti-ErbB 3 antibody images:
Western blot - ErbB 3 antibody (ab5470)
Anti-ErbB 3 antibody (ab5470) + Mouse brain lysate
Predicted band size : 148 kDa
Observed band size : 155 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 3 antibody (ab5470)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HC = hepatocarcinoma.
Protocols
References for Anti-ErbB 3 antibody (ab5470)
This product has been referenced in:
- Wan Jet al. Modification of HER2 pre-mRNA alternative splicing and its effects on breast cancer cells. Int J Cancer 124:772-7 (2009). WB; Human.Read more (PubMed: 19035464) »
- Yu WMet al. Schwann cell-specific ablation of laminin gamma1 causes apoptosis and prevents proliferation. J Neurosci 25:4463-72 (2005).Read more (PubMed: 15872093) »
See all 2 publications for this product
Publishing research using ab5470? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"