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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Anti-ErbB 3 antibody
See all ErbB 3 products (19) ...
Rabbit polyclonal to ErbB 3
ELISA, WBmore details
Reacts with
Mouse, Human
Synthetic peptide conjugated to KLH, corresponding to amino acids 41-56 of Human Erb B3.
Mouse brain lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Our Abpromise guarantee covers the use of ab5470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
Binds and is activated by neuregulins and NTAK.
Epithelial tissues and brain.
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Overexpressed in a subset of human mammary tumors.
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Secreted and Cell membrane.
Target information above from: UniProt accessionP21860
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ErbB 3 antibody (ab5470)

Anti-ErbB 3 antibody (ab5470) + Mouse brain lysate
Predicted band size : 148 kDa
Observed band size : 155 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 3 antibody (ab5470)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HC = hepatocarcinoma.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab5470? Please let us know so that we can cite the reference in this datasheet
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Anti-ErbB 3 antibody (ab5470) + Mouse brain lysate
Predicted band size : 148 kDa
Observed band size : 155 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HC = hepatocarcinoma.
0
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