ErbB 4 antibody [E200] (ab32375)

Product Name 

ErbB 4 antibody [E200]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E200] to ErbB 4

Immunogen 

Synthetic peptide corresponding to the C terminus of human ErbB 4.

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Specificity 

This antibody is specific to ErbB 4. It does not cross react with other EGF receptor family members.

Tested applications 
(see key)

Flow Cyt, ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/40.
ICC: 1/50.
IP: 1/100.
WB: 1/4000. Predicted molecular weight: 147 kDa.

Is unsuitable for IHC.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 cell lysate.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu.

ErbB 4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E200

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

References for ErbB 4 antibody [E200] (ab32375)

  This product has been used in: (two most recent references)

Hollm n M et al. Suppression of breast cancer cell growth by a monoclonal antibody targeting cleavable ErbB4 isoforms. Oncogene 28:1309-19 (2009). WB; Human. PubMed: 19151766

Tvorogov D et al. Somatic mutations of ErbB4: selective loss-of-function phenotype affecting signal transduction pathways in cancer. J Biol Chem 284:5582-91 (2009). WB; Human. PubMed: 19098003

See all 4 publication references for this product.


If you publish research using ab32375 please let us know so that we can cite the reference on this datasheet.

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