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Product Name
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ErbB 4 antibody [H4.77.16]
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See all ErbB 4 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [H4.77.16] to ErbB 4
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Immunogen
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Extracellular fragment of recombinant human c-erbB-4 / HER 4 oncoprotein.
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Reacts with
(species key)
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Hu, Ms, Rat
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Epitope
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Extracellular domain.
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Tested applications
(see key)
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Flow Cyt, FuncS, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cytometry: assay dependent.
Functional studies (see below): assay dependent.
Immunoprecipitation (with protein G): use at a concentration of 2µg/mg of protein lysate.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
This antibody is not suitable for western blot (Tang C, et. al).
This antibody does not interfere with receptor-heregulin binding and is particularly wellsuited for immunoprecipitation of unoccupied as wellas the occupied c-erbB-4 receptor (Chen X, et. al.).
It reacts withintact cells, stimulates tyrosine kinase activity, andefficiently stimulates internalization of c-erbB-4 (Chen X, et. al.).
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Positive control
(see definition)
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T47D or MDA-MB-453 cells.
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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Relevance
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Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu.
ErbB 4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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H4.77.16
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ErbB 4 antibody [H4.77.16] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ErbB 4
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