Anti-ErbB 4 antibody (ab63354)

Please suggest sopme ErbB4 antibodies that will detect the extracellular domain. For human samples and ICC, live and fixed cells.

ANSWER:

Thank you for your telephone call earlier today.

According to Swissprot/Uniprot protein database site, the extracellular domain of the ErbB 4 is from amino acids 26 – 651.

http://www.uniprot.org/uniprot/Q15303

We do have the following ErbB4 antibodies tested in ICC-If and human, but regrettably, their immunogen seuqences are all in the cytoplasmic domain. They have been tested in ICC on fixed cells. As discussed on the telephone, I am sorry they will not have been tested and guarnateed for live cell imaging. I am copying the details below in case they are still of interest to you. I can recommend to review the online datasheets for further information.

ab19391 Anti-ErbB 4 antibody [HFR1] Mouse monoclonal Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse Immunogen: RSTLQHPDYLQEYST amino acids 1250-1264 Click here (or use the following: http://www.abcam.com/index.html?datasheet=19391).

ab109273 Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] Rabbit monoclonal Applications: Flow Cyt, ICC/IF, WB Reacts with: Human, Mouse, Rat Immunogen: residues surrounding phosphorylated Tyrosine 1284 Click here (or use the following: http://www.abcam.com/index.html?datasheet=109273).

ab61059 Anti-ErbB 4 (phospho Y1284) antibody Rabbit polyclonal Applications: ELISA, ICC/IF, WB Reacts with: Human, Mouse, Rat Immunogen: phosphorylation site of tyrosine 1284 (P-E-YP-L-S) Click here (or use the following: http://www.abcam.com/index.html?datasheet=61059).

ab63354 Anti-ErbB 4 antibody Rabbit polyclonal Applications: ELISA, ICC/IF, IHC-P, WB Reacts with: Human Immunogen: phosphorylation site of tyrosine 1284. Click here (or use the following: http://www.abcam.com/index.html?datasheet=63354).

I am sorry we do not have antibodies that exactly meet your requirements on this occasion, but I hope this will still be helpful. If you have any further questions, please do not hesitate to contact us.