Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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All lanes : Anti-ErbB 4 (phospho Y1258) antibody [EP2272AY] (ab76132) at 1/2000 dilution
Lane 1 : SKBR3 cell lysates treated with EGF
Lane 2 : SKBR3 cell lysates untreated
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 147 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
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