ErbB 4 (phospho Y1284) antibody (ab61059)

Product Name 

ErbB 4 (phospho Y1284) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ErbB 4 (phospho Y1284)

Immunogen 

Synthetic phosphopeptide derived from human ErbB 4 around the phosphorylation site of tyrosine 1284 (P-E-Y^P-L-S)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects endogenous levels of ErbB 4 only when phosphorylated at tyrosine 1284.

Tested applications 
(see key)

ELISA, ICC/IF

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/100000.
ICC/IF: 1/100 - 1/500.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cells treated with 200nM EGF for 5 minutes.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu.

ErbB 4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺),150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody against non-phosphopeptide was removed by chromatography
using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

See below for ErbB 4 (phospho Y1284) antibody images, references, products related to ab61059 and other tools.

ErbB 4 (phospho Y1284) antibody images:

Customer reviews (feedback) regarding ErbB 4 (phospho Y1284) antibody

Customer FAQs regarding ErbB 4 (phospho Y1284) antibody

Protocols for ErbB 4 (phospho Y1284) antibody

Price and availability of products related to ErbB 4 (phospho Y1284) antibody

ErbB 4 (phospho Y1284) antibody - related products: