ErbB 4 protein (Tagged) (ab61640)
Constituents: 50% Glycerol, 0.05% Tween 20, 5mM Glutathione, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Our Abpromise guarantee covers the use of ab61640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 3.29 pmol/min/µg.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- 4ICDAvian erythroblastic leukemia viral oncogene homolog 4Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4
- E4ICDEC 18.104.22.168ErbB4ERBB4 intracellular domainERBB4_HUMANHER 4HER4Mer4MGC138404Oncogene ERBB4P180erbB4Proto-oncogene-like protein c-ErbB-4Receptor protein tyrosine kinase erbB 4 precursorReceptor tyrosine protein kinase erbB 4s80HER4Tyrosine kinase type cell surface receptor HER4Tyrosine kinase-type cell surface receptor HER4v erb a avian erythroblastic leukemia viral oncogene homolog like 4v erb a erythroblastic leukemia viral oncogene homolog 4v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4Verba avian erythroblastic leukemia viral oncogene homolog like 4Verba erythroblastic leukemia viral oncogene homolog 4VERBB2
Contains 1 protein kinase domain.
modificationsIsoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
References for ErbB 4 protein (Tagged) (ab61640)
ab61640 has not yet been referenced specifically in any publications.