Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)
- Product nameErk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell Lysate
- Assay typeSandwich (qualitative)
- Assay time5h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse, Rat, Human
- Product overview
ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysate Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187) 1 vial Detection Antibody Erk1/2 1 vial Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody 1 unit HRP-conjugated anti-rabbit IgG: 500x HRP-conjugated anti-rabbit IgG concentrate 1 x 25µl Positive Control: lyophilized powder from A431 cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Cellular localizationCytoplasmic and Nuclear
- ERK 1
- ERK 2
- extracellular signal regulated kinase 1
- extracellular signal regulated kinase 2
- extracellular signal related kinase 1
- insulin stimulated MAP2 kinase
- MAP kinase 1
- MAP kinase 2
- MAP kinase 3
- MAP kinase isoform p42
- MAP kinase isoform p44
- MAPK 1
- MAPK 2
- microtubule associated protein 2 kinase
- mitogen activated protein kinase 1
- mitogen activated protein kinase 2
- mitogen activated protein kinase 3
- p42 MAPK
- p44 ERK1
- p44 MAPK
- protein tyrosine kinase ERK2
- Entrez Gene: 5595 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 26413 Mouse
- Entrez Gene: 50689 Rat
- Entrez Gene: 116590 Rat
- Omim: 601795 Human
- Omim: 176948 Human
- SwissProt: P28482 Human
- SwissProt: P27361 Human
- SwissProt: P63085 Mouse
- SwissProt: Q63844 Mouse
- SwissProt: P63086 Rat
- SwissProt: P21708 Rat
- Unigene: 861 Human
- Unigene: 431850 Human
- Unigene: 8385 Mouse
- Unigene: 196581 Mouse
- Unigene: 2592 Rat
- Unigene: 34914 Rat
Our Abpromise guarantee covers the use of ab126445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit images
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126445.
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using Western Blot.
References for Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)
ab126445 has not yet been referenced specifically in any publications.