Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)

Overview

• Product nameErk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit
• Tests
  1 x 96 well plate
• Sample type
  Cell Lysate
• Assay typeSandwich
• Assay time
  5h 00m
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Product overview

  ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.

  This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Tested applicationsSandwich ELISA more details

Properties

• Storage instructionsStore at -20°C. Please refer to protocols.

• Components	Identifier	1 x 96 tests
  20X Wash Buffer Concentrate	Item B	1 x 25ml
  2X Cell Lysate Buffer	Item J	1 x 5ml
  Assay Diluent: 5x concentrated buffer		1 x 15ml
  Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187)		1 vial
  Detection Antibody Erk1/2		1 vial
  Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody		1 unit
  HRP-conjugated anti-rabbit IgG: 500x HRP-conjugated anti-rabbit IgG concentrate		1 x 25µl
  Positive Control: lyophilized powder from A431 cell lysate		1 vial
  Stop Solution	Item I	1 x 8ml
  TMB One-Step Substrate Reagent	Item H	1 x 12ml

• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • MAPK pathway

  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Other

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Phosphoprotein ELISA kits

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Other

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Kinases

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Other

Applications

Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit images

• 

  Sandwich ELISA - Erk1 (pT202/Y204 + Erk2 (pY185/187) + total Erk1/2 ELISA Kit (ab126445)
  A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.
• 

  Sandwich ELISA - Erk1 (pT202/Y204 + Erk2 (pY185/187) + total Erk1/2 ELISA Kit (ab126445)
  A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126445.
• 

  Western blot - Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)
  A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using Western Blot.

Protocols

Protocol Booklet