Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)
- Product nameErythropoietin (EPO) Human ELISA Kit (with color giving dyes)See all Erythropoietin (EPO) kits ...
Intra-assay Sample n Mean SD CV% Overall 7.8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity0.4 µlU/ml
- Range1.6 µlU/ml - 100 µlU/ml
Sample specific recovery Sample type Average % Range Serum 81 71% - 93%
- Species reactivityReacts with: Human
- Product overview
ab119522 Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Erythropoietin. ab119522 is for research use only and is not for diagnostic or therapeutic procedures.
Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), were tested with this assay.
In an experiment involving 9 EDTA- and 15 heparin plasma samples, as well as 15 serum samples, ab119522 and a competitor ELISA Kit show a correlation factor of R = 0.973.
Erythropoietin (EPO) is a hormone produced by the kidney that promotes the formation of red blood cells in the bone marrow. EPO is a glycoprotein with a molecular weight of 34 kDa. The kidney cells that primarily make EPO are specialized and sensitive to low oxygen levels in the blood. These cells release EPO when the oxygen level is low in the kidney.
EPO binds the Epo receptor (Epo R) on erythroid progenitors in bone marrow eliciting proliferation, maturation, and differentiation of red blood cells thereby increase the oxygen-carrying capacity of the blood.
EPO is the prime physiological regulator of red blood cell production.
The measurement of EPO in the blood is useful in the study of bone marrow disorders and kidney disease. Elevated levels of EPO can be seen in polycythemia, a disorder in which there is an excess of red blood cells. Lower than normal levels of EPO are seen in chronic renal failure.
EPO plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process. Measurement of serum immunoreactive erythropoietin has shown that overproduction of EPO can be an adaptive response to conditions producing tissue hypoxia, such as smoking chronic obstructive pulmonary disease, renal hypoxia or cyanotic heart disease. Elevated serum levels have further been found in patients suffering from various neoplastic diseases, such as renal carcinomas and benign renal tumors, liver carcinomas and hepatomas, cerebellar hemangioblastomas and other neoplastic disorders. Serum levels of EPO lower than normal are found in various forms of anemias. In case EPO is not primarily involved in the cause of the anemia, elevated levels of EPO are found in serum of the patients suffering from diseases like aplastic anemias, iron deficiency, megaloblastic anemias, thalassemias and myelodysplastic syndromes.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human Erythropoietin (EPO) monoclonal antibody 1 x 70µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Erythropoietin (EPO) Standard 2 vials Monoclonal anti-human Erythropoietin-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
Our Abpromise guarantee covers the use of ab119522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) images
Representative standard curve using ab119522.
References for Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)
ab119522 has not yet been referenced specifically in any publications.