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Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)

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Overview

  • Product nameErythropoietin (EPO) Human ELISA Kit (with color giving dyes)See all Erythropoietin (EPO) kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 7.8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    0.4 µlU/ml
  • Range
    1.6 µlU/ml - 100 µlU/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 81 71% - 93%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119522 Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Erythropoietin. ab119522 is for research use only and is not for diagnostic or therapeutic procedures.

    Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), were tested with this assay.

    In an experiment involving 9 EDTA- and 15 heparin plasma samples, as well as 15 serum samples, ab119522 and a competitor ELISA Kit show a correlation factor of R = 0.973.

    Erythropoietin (EPO) is a hormone produced by the kidney that promotes the formation of red blood cells in the bone marrow. EPO is a glycoprotein with a molecular weight of 34 kDa. The kidney cells that primarily make EPO are specialized and sensitive to low oxygen levels in the blood. These cells release EPO when the oxygen level is low in the kidney.

    EPO binds the Epo receptor (Epo R) on erythroid progenitors in bone marrow eliciting proliferation, maturation, and differentiation of red blood cells thereby increase the oxygen-carrying capacity of the blood.

    EPO is the prime physiological regulator of red blood cell production.

    The measurement of EPO in the blood is useful in the study of bone marrow disorders and kidney disease. Elevated levels of EPO can be seen in polycythemia, a disorder in which there is an excess of red blood cells. Lower than normal levels of EPO are seen in chronic renal failure.

    EPO plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process. Measurement of serum immunoreactive erythropoietin has shown that overproduction of EPO can be an adaptive response to conditions producing tissue hypoxia, such as smoking chronic obstructive pulmonary disease, renal hypoxia or cyanotic heart disease. Elevated serum levels have further been found in patients suffering from various neoplastic diseases, such as renal carcinomas and benign renal tumors, liver carcinomas and hepatomas, cerebellar hemangioblastomas and other neoplastic disorders. Serum levels of EPO lower than normal are found in various forms of anemias. In case EPO is not primarily involved in the cause of the anemia, elevated levels of EPO are found in serum of the patients suffering from diseases like aplastic anemias, iron deficiency, megaloblastic anemias, thalassemias and myelodysplastic syndromes.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionErythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
  • Tissue specificityProduced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Involvement in diseaseGenetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2) [MIM:612623]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similaritiesBelongs to the EPO/TPO family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01588 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        EPEPOEPO_HUMAN
        EpoetinErythropoietinMVCD2
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119522 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)
        Sandwich ELISA - Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)
        Representative standard curve using ab119522.

      Protocols

      References for Erythropoietin (EPO) Human ELISA Kit (with color giving dyes) (ab119522)

      ab119522 has not yet been referenced specifically in any publications.

      Publishing research using ab119522 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab119522 Submit a Question for ab119522

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      Hi. I am just wondering about the kit.

      I use the abcam ELISA kits (ab119522) to measure the EPO levels. I usually took the human serum for test, but this time I want to change the matrix from human serum to rat plasma or dog plasma.

      ...

      Read More

      Thank you for your enquiry regarding ab119522.

      This product has been designed to detect human Erythropoietin (EPO) and it has only been tested on human samples; we do not have any information regarding its cross-reactivity with other species. Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"