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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-Escargot antibody
Description
Mouse polyclonal to Escargot
Tested applications
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Fruit fly (Drosophila melanogaster)
Immunogen
Recombinant fragment: SAKKDKNQPP RYQCPDCQKS YSTFSGLTKH QQFHCPAAEG , corresponding to amino acids 298-337 of Fruit fly (Drosophila melanogaster) Escargot
SAKKDKNQPP RYQCPDCQKS YSTFSGLTKH QQFHCPAAEG
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Western blot - Escargot antibody (ab43635)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 52 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Escargot is a member of the Snail family of transcription factors required for nervous system and mesoderm development. There are 3 members of the Snail family found in Drosophila: esgcargot, snail and worniu. Escargot binds to the DNA sequence A/GCAGGTG and contains 5 zinc finger DNA binding domains. It contributes to central nervous system development and is normally found within embryonic neuroblasts.
Cellular localization
Nuclear
Alternative names
- 4B7 antibody
- br43 antibody
- dgl antibody
see all
Database links
- Entrez Gene: 34903 Fruit fly (Drosophila melanogaster)
- SwissProt: P25932 Fruit fly (Drosophila melanogaster)
- Unigene: 4764 Fruit fly (Drosophila melanogaster)
Anti-Escargot antibody images:
Western blot - Escargot antibody (ab43635)
All lanes : Anti-Escargot antibody (ab43635) at 1/1000 dilution
Lane 1 : E. coli whole cell lysate containing 50 - 100ng of fusion protein with an irrelevant antigen
Lane 2 : E. coli whole cell lysate containing 50 - 500ng of the recombinant fusion protein used as antigen
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated
Predicted band size : 52 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
The observed band size does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it's part of a fusion protein
References for Anti-Escargot antibody (ab43635)
ab43635 has not yet been referenced specifically in any publications.
Publishing research using ab43635? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"