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Estradiol 17-Beta Human ELISA Kit (ab108667)

Submit an Abreview Q&A (6)

Overview

  • Product nameEstradiol 17-Beta Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    8.68 pg/ml
  • Range
    20 pg/ml - 2000 pg/ml
  • Recovery

    101.09 %

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108667 Competitive immunoenzymatic colorimetric method for quantitative determination of Estradiol 17-beta in human serum or plasma.

    Estradiol 17-beta is a sex hormone. It represents the major Estrogen in humans. Estradiol 17-beta has not only a critical impact on reproductive and sexual functioning, but also affects other organs including bone structure. During the reproductive years most Estradiol 17-beta in women is produced by the ovaries, smaller amounts of Estradiol 17-beta are also produced by the adrenal cortex. In men, the testes produce Estradiol 17-beta. In plasma Estradiol 17-beta is largely bound to sex hormone binding globulin (SHBG), also to albumin, only a fraction is free and biologically active. Serum Estradiol 17-beta measurement in women reflects primarily the activity of the ovaries. During pregnancy v levels, including Estradiol 17-beta, rise steadily towards term. Estradiol 17-beta increases due to placental production. In adult premenopausal women, ovarian production of Estradiol 17-beta is stimulated by luteinizing hormone (LH) and the follicle-stimulating hormone (FSH) during the menstrual cycle. In adult women, Estradiol 17-beta levels are measured in the evaluation of fertility and menstrual irregularities, and to monitor ovarian follicular function during induction of ovulation. In the female, Estradiol 17-beta acts as a growth hormone for tissue of the reproductive organs. The development of secondary sexual characteristics in women is driven by Estradiol 17-beta. Estradiol 17-beta is involved also in men fertility. Estradiol 17-beta regulates the bone maintenance. Post-menopause women experience an accelerated loss of bone mass due to a relative Estradiol 17-beta deficiency. Estradiol 17-beta affects the production of multiple proteins including lipoproteins, binding proteins, and proteins responsible for blood clotting. Estrogens have been found to have neuroprotective function. The Estradiol 17-beta, for his activities, is involved in some types of cancer such as breast cancer and cancer of the uterine lining. In addition there are several benign gynaecologic conditions that are dependent on Estrogen such as endometriosis, leiomyomata uteri, and uterine bleeding.

  • Tested applicationsCompetitive ELISA more details

Properties

  • Alternative names
      Estradiol

Applications

Our Abpromise guarantee covers the use of ab108667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA cELISA

Protocols

References for Estradiol 17-Beta Human ELISA Kit (ab108667)

ab108667 has not yet been referenced specifically in any publications.

Publishing research using ab108667 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 6 of 6 results for Abreviews and Q&A

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I have serum that gives to high of a signal at full strength. I typically dilute in PBS/1% BSA. Is this dilution buffer compatible with this kit?

It is recommended to use the kit component 'Estradiol 17-beta Standard 0' to dilute high value samples. The standard is prepared from 17 Beta-Estradiol free serum. Alternatively, serum from males free of Estradiol should be use as the base to dilute high ...

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Customer has kindly contacted us regarding the expected relationship between absorbance and concentration. When running the kit they found this relationship to be inverse (the highest concentration standard had lowest absorbance value). Is this to be expe...

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Our 17 Beta-Estradiol ELISA ab08667 is a competitive immunoassay. Therefore, one would expect an inverse relationship between the absorbance and concentration.

Hi,

I wish to purchase the Estradiol 17-Beta Human ELISA Kit (ab108667), testing it in the mouse and taking advantage of the offer described below.

Can you please send me the discount code.

Many Thanks,

Thank you for your reply.

I'm pleased to hear you would like to participate in the testing discount scheme with the Estradiol 17-Beta Human ELISA Kit (ab108667). The discount code is as follows:

Discount code: xxxxxxxx

Expirat...

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Phone call requesting information on the Estradiol 17-Beta Human ELISA Kit (ab108667).

Thank you for contacting us yesterday in regards to the Estradiol 17-Beta Human ELISA Kit (ab108667).

As discussed over the phone, whilst this kit has not been tested with mouse samples specifically I would expect the kit to be able to detect th...

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Just wondering if you guys can send us an estrogen kit to try.
We do a lot of these hormonal studies and are always on the
look for a better kit. Some people in our lab do not like the
estrogen BQ kit and if your kit works, we may like to s...

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Thank you for your reply.

While we do not provide free samples of any of our products, I can offer a discount off a future purchase if youpurchase one of these ELISA kitsnow, test it in mouse and submit feedback to us in the form of an Abreview....

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I am looking for a custom elisa kit which can detect both 17 beta estradiol and
testosterone simultaneously from mouse serum.
Please do let me know if you offer something.

Thank you for contacting us.

We do not currently have any kits that would test for 17 beta estradiol and testosterone simultaneously. We have some ELISA kits that would be suitable for use on human samples, but they have not been tested for mous...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"