Estradiol E2 Human ELISA Kit (ab108640)
- Product nameEstradiol E2 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeSerum
- Assay typeCompetitive
- Sensitivity= 10 pg/ml
- Range10 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 101.3 18pg/ml - 810pg/ml
- Species reactivityReacts with: Human
- Product overview
ab108640, Estradiol E2 Human ELISA Kit is intended for the quantitative determination of Estradiol E2 in human serum.
Estradiol E2 is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Estradiol E2 is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG). To a lesser extent it is bound to other serum proteins such as albumin. Only a tiny fraction circulates as free hormone or in the conjugated form. Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinizing hormone (LH), which are essential for follicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests Estradiol E2 Control 1 1 x 0.5ml Estradiol E2 Control 2 1 x 0.5ml Estradiol E2 Reference Standard Set 6 x 0.5ml Estradiol E2-HRP Conjugate Reagent Blue colour 1 x 12ml Goat Anti-Rabbit IgG-Coated microtiter plate 1 x 96 tests Rabbit Anti-Estradiol E2 Reagent Pink colour 1 x 7ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml
- Research Areas
- 17ß estradiolE2ethynyl EstradiolOestradiol
Our Abpromise guarantee covers the use of ab108640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Estradiol E2 Human ELISA Kit (ab108640)
ab108640 has not yet been referenced specifically in any publications.