Estradiol E2 Human ELISA Kit (ab108640)
- Product nameEstradiol E2 Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% 13 pg/mL 24 3 24.1% 73 pg/mL 24 7 10.3% 247 pg/mL 24 10 4.1% 633 pg/mL 24 31 4.9%
- Tests1 x 96 well plate
- Sample typeSerum
- Assay typeCompetitive
- Sensitivity10 pg/ml
- Range10 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 101.3 18pg/ml - 810pg/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.
A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests Estradiol E2 Control 1 1 x 0.5ml Estradiol E2 Control 2 1 x 0.5ml Estradiol E2 Standard 1 - 10 pg/mL 1 x 0.5ml Estradiol E2 Standard 3 - 100 pg/mL 1 x 0.5ml Estradiol E2 Standard 4 - 300 pg/mL 1 x 0.5ml Estradiol E2 Standard 0 - 0 pg/mL 1 x 0.5ml Estradiol E2 Standard 5 - 1,000 pg/mL 1 x 0.5ml Estradiol E2-HRP Conjugate Reagent Blue colour 1 x 12ml Estradiol E2 Standard 2 - 30 pg/mL 1 x 0.5ml Goat anti-rabbit IgG Coated Microplate (12 x 8 wells) 1 x 96 tests Rabbit Anti-Estradiol E2 Reagent Pink colour 1 x 7ml Stop Solution 1 x 11ml TMB Substrate Solution 1 x 11ml
- Research Areas
- 17ß estradiol
- ethynyl Estradiol
Our Abpromise guarantee covers the use of ab108640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Estradiol E2 Human ELISA Kit (ab108640)
ab108640 has not yet been referenced specifically in any publications.