Estriol Human ELISA Kit (ab108675)
Overview
- Product nameEstriol Human ELISA KitSee all Estriol kits ...
- Detection methodColorimetric
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity1 ng/ml
- Range2 ng/ml - 200 ng/ml
- Recovery
94 %
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
ab108675 Estriol Human ELISA Kit uses a competitive immunoenzymatic colorimetric method for quantitative determination of Estriol in serum or plasma.
Estriol is a female sex steroid hormone and one of the three major naturally occurring estrogens, the others being estradiol and estrone. Estriol is produced almost exclusively during pregnancy and is the major estrogen produced in the normal human fetus. During pregnancy the production of Estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of Estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than nonpregnant levels. In non-pregnant circumstances, Estriol is derived almost exclusively from 17 ß-Estradiol. Estriol exists in biological matrix in free (ca. 9 %) and conjugated form (91 %). Conjugation with glucuronic or sulphuric acid of estriol occurs in the liver, which aides in steroid solubility, therefore allowing rapid elimination via the kidney.
- Tested applicationsELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Anti-Estriol IgG Coated Wells 12 x 8 tests Cover foils 2 units Estriol Standards 4 x 1ml Estriol-HRP conjugate conc 1 x 0.4ml Incubation buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 12ml - Research Areas
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Alternative names
- Oestriol
Applications
Our Abpromise guarantee covers the use of ab108675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Protocols
References for Estriol Human ELISA Kit (ab108675)
ab108675 has not yet been referenced specifically in any publications.