Anti-Estrogen Receptor alpha antibody [1D5] (ab17087)

Overview

• Product nameAnti-Estrogen Receptor alpha antibody [1D5]See all Estrogen Receptor alpha primary antibodies ...
• Description
  Mouse monoclonal [1D5] to Estrogen Receptor alpha
• Tested applicationsFlow Cyt, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human).

• Positive controlBreast carcinoma

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA
• Concentration information loading...
• PurityIgG fraction
• Primary antibody notes This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen.
• Clonality Monoclonal
• Clone number1D5
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Receptors

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Estrogen

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

Applications

Target

• FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• Post-translational
  modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
  Glycosylated; contains N-acetylglucosamine, probably O-linked.
  Ubiquitinated. Deubiquitinated by OTUB1.
  Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
  Palmitoylated (isoform 3). Not biotinylated (isoform 3).
• Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

Target information above from: UniProt accession P03372 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2099 Human
  • Omim: 133430 Human
  • SwissProt: P03372 Human
  • Unigene: 208124 Human

• Alternative names
  DKFZp686N23123 antibodyER alpha antibodyER antibody

  ER-alpha antibodyERA antibodyESR 1 antibodyESR antibodyESR1 antibodyESR1_HUMAN antibodyESRA antibodyEstradiol receptor antibodyEstrogen nuclear receptor alpha antibodyEstrogen receptor 1 antibodyEstrogen receptor alpha 3*,4,5,6,7*/822 isoform antibodyEstrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibodyEstrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibodyEstrogen receptor alpha delta 4 +49 isoform antibodyEstrogen receptor alpha delta 4*,5,6,7*/654 isoform antibodyEstrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibodyEstrogen receptor antibodyNR3A 1 antibodyNR3A1 antibodyNuclear receptor subfamily 3 group A member 1 antibody

  see all

Anti-Estrogen Receptor alpha antibody [1D5] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [1D5] (ab17087)
  ab17087 staining Estrogen Receptor alpha in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Estrogen Receptor antibody [1D5] (ab17087)
  Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor using ABC and DAB chromogen.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Receptor antibody [1D5] (ab17087)
  ab17087 staining human breast carcinoma by IHC-P.
• 

  Flow Cytometry-Anti-Estrogen Receptor alpha antibody [1D5](ab17087)
  Overlay histogram showing MCF7 cells stained with ab17087 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17087, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.