Anti-Estrogen Receptor alpha antibody (ab3575)

Overview

• Product nameAnti-Estrogen Receptor alpha antibodySee all Estrogen Receptor alpha primary antibodies ...
• Description
  Rabbit polyclonal to Estrogen Receptor alpha
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Synthetic peptide: N(21)ELEPLNRPQLK(32)C. Immunizing peptide corresponds to amino acid residues 21-32 from human ER alpha. This peptide sequence is completely conserved between human, rat, mouse, rabbit, sheep, porcine and bovine species.

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• Positive controlICC: Human coronary artery smooth muscle cells (See Han et al) This antibody gave a positive result in IHC in the following FFPE tissue: Human breast Ductal carcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS, 1 mg/ml BSA and 0.05% sodium azide
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Estrogen

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

Applications

Target

• FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• Post-translational
  modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
  Glycosylated; contains N-acetylglucosamine, probably O-linked.
  Ubiquitinated. Deubiquitinated by OTUB1.
  Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
  Palmitoylated (isoform 3). Not biotinylated (isoform 3).
• Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

Target information above from: UniProt accession P03372 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2099 Human
  • Entrez Gene: 24890 Rat
  • Omim: 133430 Human
  • SwissProt: P03372 Human
  • SwissProt: P06211 Rat
  • Unigene: 208124 Human
  • Unigene: 10595 Rat

• Alternative names
  DKFZp686N23123 antibodyER alpha antibodyER antibody

  ER-alpha antibodyEra antibodyESR 1 antibodyESR 1 antibodyESR antibodyESR1 antibodyESR1 antibodyESR1_HUMAN antibodyESRA antibodyESRA antibodyEstradiol receptor antibodyEstradiol receptor antibodyEstrogen nuclear receptor alpha antibodyEstrogen receptor 1 antibodyEstrogen receptor alpha 3*,4,5,6,7*/822 isoform antibodyEstrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibodyEstrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibodyEstrogen receptor alpha delta 4 +49 isoform antibodyEstrogen receptor alpha delta 4*,5,6,7*/654 isoform antibodyEstrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibodyEstrogen receptor antibodyMajor ORF antibodyNR3A1 antibodyNR3A1 antibodyNuclear receptor subfamily 3 group A member 1 antibody

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Anti-Estrogen Receptor alpha antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (ab3575)

  ab3575 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3575 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  - Estrogen Receptor alpha antibody (ab3575)
  ab3575 at a dilution of 1-5µg/ml staining ER alpha in rat prostate.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)

  IHC image of Estrogen Receptor alpha staining in Human breast Ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3575, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

• Recommended ICC protocol with ab3575 and ab3577