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Product name
Anti-Estrogen Receptor alpha antibody
See all Estrogen Receptor alpha products (49) ...
Description
Rabbit polyclonal to Estrogen Receptor alpha
Specificity
This antibody antibody detects endogenous levels of total Estrogen Receptor alpha protein.
Tested applications
ICC/IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
The antiserum was produced against synthesized non phosphopeptide derived from human Estrogen Receptor alpha around the phosphorylation site of serine 106.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
Target information above from: UniProt accessionP03372
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
see all
Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- Unigene: 208124 Human
- Unigene: 463262 Mouse
- Unigene: 9213 Mouse
Anti-Estrogen Receptor alpha antibody images:
Western blot - Estrogen Receptor alpha antibody (ab39642)
Predicted band size : 68 kDa
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor alpha antibody (ab39642) and Estrogen Receptor alpha (phospho-Ser106) antibody.
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha antibody (ab39642)
Ab39642 staining human Estrogen Receptor alpha in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence-Estrogen Receptor alpha antibody(ab39642)
ICC/IF image of ab39642 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39642, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Estrogen Receptor alpha antibody (ab39642)
ab39642 has not yet been referenced specifically in any publications.
Publishing research using ab39642? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"