Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Secondary antibodies
- Slides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Estrogen Receptor alpha (phospho S167) antibody
See all Estrogen Receptor alpha products (49) ...
Description
Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
Specificity
ab31478 detects endogenous levels of estrogen receptor alpha only when phosphorylated at Serine 167.
Tested applications
IHC-P, WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Serine 167
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
-
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
(enlarge)
-
Western blot - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
Target information above from: UniProt accessionP03372
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
see all
Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
Anti-Estrogen Receptor alpha (phospho S167) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab31478 at 1/50. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Western blot - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) at 1/500 dilution
Lane 1 : HeLa cells treated with EGF
Lane 2 : Untreated HeLa cell lysate
Predicted band size : 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Receptor alpha (phospho S167) antibody (ab31478)
ab31478 staining human non-small cell lung cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in 10 mM sodium citrate (pH 6) prior to blocking with a commercially available blocking reagent for 20 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 24 hours at 4°C. A HRP conjugated antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)
This product has been referenced in:
- Skliris GP et al. A phosphorylation code for oestrogen receptor-alpha predicts clinical outcome to endocrine therapy in breast cancer. Endocr Relat Cancer 17:589-97 (2010).Read more (PubMed: 20418363) »
- Márquez-Garbán DC et al. Estrogen receptor signaling pathways in human non-small cell lung cancer. Steroids 72:135-43 (2007).Read more (PubMed: 17276470) »
See all 2 publications for this product
Publishing research using ab31478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"