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Estrogen Receptor alpha protein (ab38041)

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Overview

  • Product nameEstrogen Receptor alpha proteinSee all Estrogen Receptor alpha proteins and peptides ...
  • Protein descriptionRecombinant human ER a protein encoding 1-300aa expressed in E.coli
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 50mM Tris acetate, 1mM EDTA, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab38041 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    ELISA
    Inhibition Assay
  • Application notesELISA: Use at an assay dependent dilution.
    Inhib: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    On SDS-PAGE (Commassie blue stained gel), the purified recombinant protein shows a band at 59 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DKFZp686N23123ERER alpha
        ER-alphaEraESRESR 1ESR 1ESR1ESR1ESR1_HUMANESRAESRAEstradiol receptorEstradiol receptorEstrogen nuclear receptor alphaEstrogen receptorEstrogen receptor 1Estrogen receptor alpha 3*,4,5,6,7*/822 isoformEstrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoformEstrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoformEstrogen receptor alpha delta 4 +49 isoformEstrogen receptor alpha delta 4*,5,6,7*/654 isoformEstrogen receptor alpha delta 4*,5,6,7,8*/901 isoformMajor ORFNR3A1NR3A1Nuclear receptor subfamily 3 group A member 1
      see all
  • FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

    • Target information above from: UniProt accession P03372 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Estrogen Receptor alpha protein (ab38041)

    ab38041 has not yet been referenced specifically in any publications.

    Publishing research using ab38041 ? Please let us know so that we can cite the reference in this datasheet

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    We are looking into if Abcam can supply this protein (ERa)in a PBS buffer solution or in a lyophilized form. The experiment to be conducted can not have any Tris or Azide in the buffer. This is a research project and we need only 10ug of this protein. ...

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    Thank you for your enquiry. I have checked with the originator of the product and unfortunately, this product does not come without Tris and Azide in the buffer. I am sorry we can't be of more help in this occasion but if there is anything else tha...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"