![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [SP1] (FITC) (ab27614) image](#Estrogen-Receptor-Primary-antibodies-ab27614-1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Estrogen Receptor antibody [SP1] (FITC)
See all Estrogen Receptor products (15) ...
Description
Rabbit monoclonal [SP1] to Estrogen Receptor (FITC)
Conjugation
FITC
Specificity
Ab27614 recognises estrogen receptor.
Tested applications
IHC-P, ICC/IF, Flow Cyt, IFmore details
Cross reactivity
Reacts with
Rat, Hamster, Human
Immunogen
Synthetic peptide derived from C terminal of human estrogen receptor.
Epitope
Ab27614 reacts with an epitope located in the C terminal region of estrogen receptor.
Positive control
Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
Ab27614 strongly stains the nucleus of epithelial cells in breast carcinomas.
Clonality
Monoclonal
Clone number
SP1
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [SP1] (FITC) (ab27614)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [SP1] (FITC) (ab27614) image](/ps/datasheet/images/27/ab27614/Estrogen-Receptor-Primary-antibodies-ab27614-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution. PubMed: 21098263
Flow Cyt: Use at an assay dependent dilution. PubMed: 21098263
IF
IF: 1/100.IF: 1/100.
Target
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
Target information above from: UniProt accessionP03372
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Atherosclerosis, susceptibility to, included antibody
- DKFZp686N23123 antibody
- ER Alpha antibody
see all
Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 24890 Rat
- Omim: 601663 Human
- SwissProt: Q9QZJ5 Hamster
- SwissProt: P03372 Human
- SwissProt: P06211 Rat
- Unigene: 208124 Human
- Unigene: 10595 Rat
Anti-Estrogen Receptor antibody [SP1] (FITC) images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [SP1] (FITC) (ab27614)
IHC image of ab27614 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27614, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Estrogen Receptor antibody [SP1] (FITC) (ab27614)
This product has been referenced in:
- Huang Z et al. Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections. Appl Immunohistochem Mol Morphol 13:91-5 (2005).Read more (PubMed: 15722800) »
- Rossi S et al. Rabbit monoclonal antibodies: a comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies. Am J Clin Pathol 124:295-302 (2005).Read more (PubMed: 16040303) »
See all 3 publications for this product
Publishing research using ab27614? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"