![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Estrogen Receptor antibody [SP1] (ab27595) image](#ab27595_1.bmp)
![Western blot - Estrogen Receptor antibody [SP1], prediluted (ab27595) image](#Estrogen-Receptor-Primary-antibodies-ab27595-1.jpg)
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Product name
Anti-Estrogen Receptor antibody [SP1], prediluted
See all Estrogen Receptor products (15) ...
Description
Rabbit monoclonal [SP1] to Estrogen Receptor, prediluted
Specificity
ab27595 recognises estrogen receptor (ER).
Tested applications
WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Hamster
Immunogen
Synthetic peptide derived from C-terminal of human estrogen receptor.
Positive control
Breast carcinomas
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6
Concentration
Concentration information loading...
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
SP1
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. PubMed: 19329436
IHC-Fr: Use at an assay dependent dilution. PubMed: 19329436
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.USE NEAT.
Target
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
Target information above from: UniProt accessionP03372
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Atherosclerosis, susceptibility to, included antibody
- DKFZp686N23123 antibody
- ER Alpha antibody
see all
Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 24890 Rat
- Omim: 601663 Human
- SwissProt: Q9QZJ5 Hamster
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- SwissProt: P06211 Rat
see all
Anti-Estrogen Receptor antibody [SP1], prediluted images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Estrogen Receptor antibody [SP1] (ab27595)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Estrogen Receptor antibody [SP1] (ab27595)](/ps/datasheet/Images/27/ab27595/ab27595_1.bmp)
Staining of human breast carcinoma using ab27595
Western blot - Estrogen Receptor antibody [SP1], prediluted (ab27595)
![Western blot - Estrogen Receptor antibody [SP1], prediluted (ab27595)](/ps/datasheet/images/27/ab27595/Estrogen-Receptor-Primary-antibodies-ab27595-1.jpg)
Anti-Estrogen Receptor antibody [SP1], prediluted (ab27595) at 1/25 dilution + lysate prepared from MCF7 cells
Image produced using the diluted version of this antibody, ab16660.
References for Anti-Estrogen Receptor antibody [SP1], prediluted (ab27595)
This product has been referenced in:
- Cao G et al. Regulation of the epithelial Mg2+ channel TRPM6 by estrogen and the associated repressor protein of estrogen receptor activity (REA). J Biol Chem 284:14788-95 (2009). WB, IHC-Fr; Human, Mouse.Read more (PubMed: 19329436) »
See 1 publication for this product
Publishing research using ab27595? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"