Recombinant Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
- Suitable for: WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Related Receptor alpha antibody [EPR46Y]
See all Estrogen Related Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR46Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
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IP |
1/20.
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Flow Cyt |
1/20.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
IP
1/20. |
Flow Cyt
1/20. |
Target
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Function
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: P11474 Human
- SwissProt: O08580 Mouse
- SwissProt: Q5QJV7 Rat
- Unigene: 110849 Human
see all -
Alternative names
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
Images
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All lanes : Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
ab76228 used at 1:20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+): HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Estrogen Related Receptor alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab76228 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab76228 was shown to specifically react with Estrogen Related Receptor alpha in wild-type HAP1 cells as signal was lost in Estrogen Related Receptor alpha knockout cells. Wild-type and Estrogen Related Receptor alpha knockout samples were subjected to SDS-PAGE. Ab76228 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab76228 has been referenced in 36 publications.
- Sakamoto T et al. The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation. Nat Commun 13:1991 (2022). PubMed: 35418170
- Li J et al. Dietary L-carnitine regulates liver lipid metabolism via simultaneously activating fatty acid β-oxidation and suppressing endoplasmic reticulum stress in large yellow croaker fed with high-fat diets. Br J Nutr N/A:1-34 (2022). PubMed: 35473947
- Rahbani JF et al. ADRA1A-Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP. Nat Metab 4:1459-1473 (2022). PubMed: 36344764
- Vangrieken P et al. Hypoxia-induced mitochondrial abnormalities in cells of the placenta. PLoS One 16:e0245155 (2021). PubMed: 33434211
- Yan H et al. Adeno-associated virus-mediated delivery of anti-miR-199a tough decoys attenuates cardiac hypertrophy by targeting PGC-1alpha. Mol Ther Nucleic Acids 23:406-417 (2021). PubMed: 33473326