Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Overview

• Product nameAnti-Estrogen Related Receptor alpha antibody [EPR46Y]See all Estrogen Related Receptor alpha primary antibodies ...
• Description
  Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
• Tested applicationsWB, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the N terminus of human Estrogen Related Receptor alpha

• Positive controlHeLa cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR46Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Estrogen

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionBinds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• Post-translational
  modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
  Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
• Cellular localizationNucleus.

Target information above from: UniProt accession P11474 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2101 Human
  • Entrez Gene: 26379 Mouse
  • Entrez Gene: 293701 Rat
  • Omim: 601998 Human
  • SwissProt: P11474 Human
  • SwissProt: O08580 Mouse
  • SwissProt: Q5QJV7 Rat
  • Unigene: 110849 Human

  • Unigene: 386776 Mouse
  • Unigene: 130171 Rat

  see all

• Alternative names
  Err 1 antibodyERR a antibodyERR alpha antibody

  ERR-alpha antibodyErr1 antibodyERR1 protein antibodyERR1_HUMAN antibodyERRa antibodyERRalpha antibodyESRL 1 antibodyESRL1 antibodyESRR A antibodyESRRA antibodyEstrogen receptor like 1 antibodyEstrogen receptor related 1 antibodyestrogen receptor related receptor alpha antibodyEstrogen receptor-like 1 antibodyEstrogen related receptor alpha antibodyEstrogen-related receptor alpha antibodyEstrra antibodyhERR1 antibodyNr3b1 antibodyNuclear receptor subfamily 3 group B member 1 antibodySteroid hormone receptor ERR1 antibody

  see all

Anti-Estrogen Related Receptor alpha antibody [EPR46Y] images

• 

  Western blot - Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/5000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 55 kDa
  Observed band size : 55 kDa
  
• 

  Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228.
  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
  Band: Band: 55kDa: Estrogen Related Receptor alpha.