RabMAb

Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Overview

  • Product nameAnti-Estrogen Related Receptor alpha antibody [EPR46Y]
    See all Estrogen Related Receptor alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
  • Tested applicationsWB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of human Estrogen Related Receptor alpha

  • Positive control
    • HeLa cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



    Ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Applications

Our Abpromise guarantee covers the use of ab76228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
Flow Cyt 1/20.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionBinds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
      Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Err 1 antibody
      • ERR a antibody
      • ERR alpha antibody
      • ERR-alpha antibody
      • Err1 antibody
      • ERR1 protein antibody
      • ERR1_HUMAN antibody
      • ERRa antibody
      • ERRalpha antibody
      • ESRL 1 antibody
      • ESRL1 antibody
      • ESRR A antibody
      • ESRRA antibody
      • Estrogen receptor like 1 antibody
      • Estrogen receptor related 1 antibody
      • estrogen receptor related receptor alpha antibody
      • Estrogen receptor-like 1 antibody
      • Estrogen related receptor alpha antibody
      • Estrogen-related receptor alpha antibody
      • Estrra antibody
      • hERR1 antibody
      • Nr3b1 antibody
      • Nuclear receptor subfamily 3 group B member 1 antibody
      • Steroid hormone receptor ERR1 antibody
      see all

    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] images

    • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: Band: 55kDa: Estrogen Related Receptor alpha.

    References for Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

    This product has been referenced in:
    • Krzysik-Walker SM  et al. The Biarylpyrazole compound AM251 alters mitochondrial physiology via proteolytic degradation of ERRa. Mol Pharmacol 83:157-66 (2013). Read more (PubMed: 23066093) »
    • Li Y  et al. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) signaling regulates mitochondrial biogenesis and respiration via estrogen-related receptor a (ERRa). J Biol Chem 288:25007-24 (2013). WB, IP ; Mouse . Read more (PubMed: 23836899) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (mammary epithelial)
    Loading amount 80 µg
    Specification mammary epithelial
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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    Submitted Jun 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"