ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC or IHC-P.
FunctionBinds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Post-translational modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity. Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
Nuclear receptor subfamily 3 group B member 1 antibody
Steroid hormone receptor ERR1 antibody
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] images
Western blot - Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 55 kDa Observed band size : 55 kDa
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: Band: 55kDa: Estrogen Related Receptor alpha.
References for Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
This product has been referenced in:
Han L et al. MicroRNA-497 downregulation contributes to cell proliferation, migration, and invasion of estrogen receptor alpha negative breast cancer by targeting estrogen-related receptor alpha. Tumour BiolN/A:N/A (2016).
Read more (PubMed: 27456360) »
Krzysik-Walker SM et al. The Biarylpyrazole compound AM251 alters mitochondrial physiology via proteolytic degradation of ERRa. Mol Pharmacol83:157-66 (2013).
Read more (PubMed: 23066093) »