RabMAb

Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Overview

  • Product nameAnti-Estrogen Related Receptor alpha antibody [EPR46Y]
    See all Estrogen Related Receptor alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
  • Tested applicationsWB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of human Estrogen Related Receptor alpha

  • Positive control
    • HeLa cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab76228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
Flow Cyt 1/20.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionBinds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
      Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Err 1 antibody
      • ERR a antibody
      • ERR alpha antibody
      • ERR-alpha antibody
      • Err1 antibody
      • ERR1 protein antibody
      • ERR1_HUMAN antibody
      • ERRa antibody
      • ERRalpha antibody
      • ESRL 1 antibody
      • ESRL1 antibody
      • ESRR A antibody
      • ESRRA antibody
      • Estrogen receptor like 1 antibody
      • Estrogen receptor related 1 antibody
      • estrogen receptor related receptor alpha antibody
      • Estrogen receptor-like 1 antibody
      • Estrogen related receptor alpha antibody
      • Estrogen-related receptor alpha antibody
      • Estrra antibody
      • hERR1 antibody
      • Nr3b1 antibody
      • Nuclear receptor subfamily 3 group B member 1 antibody
      • Steroid hormone receptor ERR1 antibody
      see all

    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] images

    • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: Band: 55kDa: Estrogen Related Receptor alpha.

    References for Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

    This product has been referenced in:
    • Krzysik-Walker SM  et al. The Biarylpyrazole compound AM251 alters mitochondrial physiology via proteolytic degradation of ERRa. Mol Pharmacol 83:157-66 (2013). Read more (PubMed: 23066093) »
    • Li Y  et al. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) signaling regulates mitochondrial biogenesis and respiration via estrogen-related receptor a (ERRa). J Biol Chem 288:25007-24 (2013). WB, IP ; Mouse . Read more (PubMed: 23836899) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (mammary epithelial)
    Loading amount 80 µg
    Specification mammary epithelial
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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    Verified customer

    Submitted Jun 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"