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Overview

  • Product nameExodus 2 Mouse ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.88 86% - 107%
    Serum 95.51 84% - 105%
    Plasma 94.29 83% - 102%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100667 Exodus 2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Exodus 2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Mouse Exodus 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Exodus 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Exodus 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Exodus 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    200X HRP-Streptavidin Concentrate Item G 1 x 200µl
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Exodus 2: biotinylated anti-mouse Exodus 2 2 vials
    Exodus 2 Microplate (12 strips x 8 wells) coated with anti-mouse Exodus 2 1 unit
    Exodus 2 standard Mouse(recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceExodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
    • Alternative names
        6CkineBeta chemokine exodus 2C-C motif chemokine 21
        Chemokine (C-C motif) ligand 21CKb9ECLEfficient Chemoattractant for LymphocytesExodus2MGC34555OTTHUMP00000000526 OTTHUMP00000000527OTTHUMP00000021303SCYA 21SCYA21Secondary lymphoid tissue chemokineSLCSmall inducible cytokine A21Small inducible cytokine subfamily A (Cys-Cys), member 21TCA 4TCA4
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100667 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Exodus 2 Mouse ELISA Kit images

      • ELISA - Exodus 2 Mouse ELISA Kit (ab100667)
        ELISA - Exodus 2 Mouse ELISA Kit (ab100667)

      Protocols

      References for Exodus 2 Mouse ELISA Kit (ab100667)

      ab100667 has not yet been referenced specifically in any publications.

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